caa a22 4500 445310138 CHVBK 20180317142626.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00404-010-1708-6 doi (NATIONALLICENCE)springer-10.1007/s00404-010-1708-6 The HER2 gene copies per tumor cell either before or after correction for chromosome-17 correlated significantly with HER2 IHC results in epithelial ovarian cancer in a tissue microarray study [Elektronische Daten] [Wen-Chih Tsai, Ming-Yung Lee, Fong-Lin Chen, Po-Hui Wang, Wea-Lung Lin, Alexandra Ruan, Yi-Ju Li, Shao-Chuan Wang, Hung Chiang, Chih-Ping Han] Background: HER2 gene amplification and HER2 protein overexpression are important factors in predicting clinical sensitivity to anti-HER2 monoclonal antibody therapy in breast cancer patients. The purpose of this study is to evaluate the correlation between HER2 protein expressions and the HER2 gene copies per tumor cell either before or after chromosome-17 correction in epithelial ovarian cancer (EOC). Methods: Adopting 2007 ASCO/CAP guideline recommendations for HER2 testing, 27 tissue microarray (TMA) samples from EOC patients were analyzed by immunohistochemistry (IHC) using Dako, c-erb-B2 antibody and subsequently examined by fluorescence in situ hybridization (FISH) using Abbott/Vysis, PathVysion HER2 DNA Probe Kit. Results: The overall concordance revealed 81.5% between HER2 IHC and HER2 FISH results. Additionally, HER2 gene copies prior to chromosome-17 correction increased significantly in a stepwise order through the negative, equivocal, and positive IHC result categories (P=0.026), as did the HER2 gene copies after chromosome-17 correction (P=0.028). On the other hand, HER2 IHC results correlated significantly with both chromosome-17 uncorrected HER2 gene copy numbers (ρ=0.430, P=0.025) and chromosome-17 corrected HER2 gene copy numbers (ρ=0.524, P=0.027). Conclusion: We demonstrated that both chromosome-17 corrected and uncorrected HER2 gene copies correlated significantly with HER2 IHC result categories; and tests for the HER2 gene copies per tumor cell either before or after correction for chromosome-17 can be applied as a potentially valuable tool in analyzing the HER2 status in EOC. Springer-Verlag, 2010 Ovarian nationallicence HER2 protein nationallicence HER2 gene nationallicence Chromosome-17 nationallicence EOC : Epithelial ovarian cancer nationallicence TMA : Tissue microarray nationallicence IHC : Immunohistochemistry nationallicence FISH : Fluorescence in situ hybridization nationallicence Polysomy-17 : Chromosome-17 polysomy nationallicence CEP17 : Chromosome-17 centromere control probe nationallicence ASCO/CAP : American Society of Clinical Oncology/College of American Pathologists nationallicence Tsai Wen-Chih Department of Obstetrics and Gynecology, Po-Jen General Hospital, Taipei, Taiwan aut Lee Ming-Yung Department of Statistics and Informatics Science, Providence University, Taichung, Taiwan aut Chen Fong-Lin Division of Pediatric Cardiology, Department of Pediatrics, Chung-Shan Medical University Hospital and School of Medicine, Chung-Shan Medical University, Taichung, Taiwan aut Wang Po-Hui Department of Obstetrics and Gynecology, School of Medicine, Chung-Shan Medical University and Chung-Shan Medical University Hospital, Taichung, Taiwan aut Lin Wea-Lung Department of Pathology, School of Medicine, Chung-Shan Medical University and Chung-Shan Medical University Hospital, Taichung, Taiwan aut Ruan Alexandra Krieger School of Arts and Sciences, Johns Hopkins University, MD, Baltimore, USA aut Li Yi-Ju Department of Pathology, School of Medicine, Chung-Shan Medical University and Chung-Shan Medical University Hospital, Taichung, Taiwan aut Wang Shao-Chuan Department of Urology, Chung-Shan Medical University Hospital, Taichung, Taiwan aut Chiang Hung Taipei Institute of Pathology, Taipei, Taiwan aut Han Chih-Ping Department of Obstetrics and Gynecology, School of Medicine, Chung-Shan Medical University and Chung-Shan Medical University Hospital, Taichung, Taiwan aut Archives of Gynecology and Obstetrics Springer-Verlag 284/3(2011-09-01), 721-729 0932-0067 284:3<721 2011 284 404 https://doi.org/10.1007/s00404-010-1708-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-010-1708-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tsai Wen-Chih Department of Obstetrics and Gynecology, Po-Jen General Hospital, Taipei, Taiwan aut NATIONALLICENCE 700 1- Lee Ming-Yung Department of Statistics and Informatics Science, Providence University, Taichung, Taiwan aut NATIONALLICENCE 700 1- Chen Fong-Lin Division of Pediatric Cardiology, Department of Pediatrics, Chung-Shan Medical University Hospital and School of Medicine, Chung-Shan Medical University, Taichung, Taiwan aut NATIONALLICENCE 700 1- Wang Po-Hui Department of Obstetrics and Gynecology, School of Medicine, Chung-Shan Medical University and Chung-Shan Medical University Hospital, Taichung, Taiwan aut NATIONALLICENCE 700 1- Lin Wea-Lung Department of Pathology, School of Medicine, Chung-Shan Medical University and Chung-Shan Medical University Hospital, Taichung, Taiwan aut NATIONALLICENCE 700 1- Ruan Alexandra Krieger School of Arts and Sciences, Johns Hopkins University, MD, Baltimore, USA aut NATIONALLICENCE 700 1- Li Yi-Ju Department of Pathology, School of Medicine, Chung-Shan Medical University and Chung-Shan Medical University Hospital, Taichung, Taiwan aut NATIONALLICENCE 700 1- Wang Shao-Chuan Department of Urology, Chung-Shan Medical University Hospital, Taichung, Taiwan aut NATIONALLICENCE 700 1- Chiang Hung Taipei Institute of Pathology, Taipei, Taiwan aut NATIONALLICENCE 700 1- Han Chih-Ping Department of Obstetrics and Gynecology, School of Medicine, Chung-Shan Medical University and Chung-Shan Medical University Hospital, Taichung, Taiwan aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 284/3(2011-09-01), 721-729 0932-0067 284:3<721 2011 284 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer