caa a22 4500 445310308 CHVBK 20180317142627.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00404-010-1771-z doi (NATIONALLICENCE)springer-10.1007/s00404-010-1771-z Evaluation of two commercialised in situ hybridisation assays for detecting HPV-DNA in formalin-fixed, paraffin-embedded tissue [Elektronische Daten] [Maresa Montag, Thomas Blankenstein, Naim Shabani, Ansgar Brüning, Ioannis Mylonas] Introduction: The role of human papilloma virus (HPV) in the pathogenesis of anogenital dysplasia is now conclusive. However, HPV detection in formalin-fixed and paraffin-embedded tissues remains controversial. Therefore, the aim of this study was to evaluate morphological changes directly in tissue specimens using a HPV-DNA detection system involving HPV in situ hybridisation. Materials and methods: Samples from patients with cervical carcinoma were analysed using the GenPoint HPV DNA Probe Cocktail (Dako, Glostrup, Denmark) and the ZytoFast HPV Screening CISH-Kit (Zytomed, Berlin, Germany). Three cervical carcinoma cell lines with a well-defined HPV copy number per cell (SiHa, HeLa, and CaSki) served as positive controls for sensitivity testing, while two HPV-negative cell lines (AC-1M32, MCF-7) and brain tissue samples served as negative controls. Moreover, to assess the validity of the in situ hybridisation, the expression of HPV-16 DNA in cell lines was demonstrated by HPV-16 E6-specific PCR. Results: Both HPV-screening assays revealed strong signals of episomal and integrated HPV-DNA at a HPV copy number of more than 50copies/cell. All cervical carcinoma samples were positive in the Dako assay, which identifies 13 high-risk HPV genotypes, whereas HPV-DNA could be detected in 9/10 cervical carcinoma samples using the Zytofast assay, identifying HPV 16, 18, 31, 33, and 35. Conclusion: HPV in situ hybridisation is a convenient and powerful tool for detecting HPV-DNA in formalin-fixed and paraffin-embedded tissue samples. Therefore, this technique is suitable for analysis of a potential HPV infection using archival pathological slides. Springer-Verlag, 2010 HPV nationallicence In situ hybridisation nationallicence Paraffin-embedded tissue nationallicence Montag Maresa First Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University, Maistrasse 11, 80337, Munich, Germany aut Blankenstein Thomas First Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University, Maistrasse 11, 80337, Munich, Germany aut Shabani Naim Department of Obstetrics and Gynecology, Klinikum Neuperlach, Munich, Germany aut Brüning Ansgar First Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University, Maistrasse 11, 80337, Munich, Germany aut Mylonas Ioannis First Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University, Maistrasse 11, 80337, Munich, Germany aut Archives of Gynecology and Obstetrics Springer-Verlag 284/4(2011-10-01), 999-1005 0932-0067 284:4<999 2011 284 404 https://doi.org/10.1007/s00404-010-1771-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-010-1771-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Montag Maresa First Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University, Maistrasse 11, 80337, Munich, Germany aut NATIONALLICENCE 700 1- Blankenstein Thomas First Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University, Maistrasse 11, 80337, Munich, Germany aut NATIONALLICENCE 700 1- Shabani Naim Department of Obstetrics and Gynecology, Klinikum Neuperlach, Munich, Germany aut NATIONALLICENCE 700 1- Brüning Ansgar First Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University, Maistrasse 11, 80337, Munich, Germany aut NATIONALLICENCE 700 1- Mylonas Ioannis First Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University, Maistrasse 11, 80337, Munich, Germany aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 284/4(2011-10-01), 999-1005 0932-0067 284:4<999 2011 284 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer