caa a22 4500 445310472 CHVBK 20180317142628.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00404-010-1765-x doi (NATIONALLICENCE)springer-10.1007/s00404-010-1765-x Up-regulated monoamine oxidase in the mouse uterus during the peri-implantation period [Elektronische Daten] [Dan Zhang, Caixia Lei, Wei Zhang] Purpose: Monoamine oxidases (MAO), functioning as the metabolism of neuroamines, have been reported to be required for endometrial receptivity recently. The aim of this study was to examine the expression patterns of the two subtypes, MAOA and MAOB, during the peri-implantation period and to investigate whether MAOA or MAOB is a useful marker for receptivity. Methods: A total of 30 uteri were collected from females of gestational day 0, 2, 4, 6, 8, and mRNA and protein expression of MAOA/B were examined by real-time PCR, Western blot analysis and Immunohistochemistry analysis, respectively. Results: We found that the mRNA of MAOA expressed in the uteri at all stages of peri-implantation and began to rise on day 4 with a continuous increase up to day 8 of pregnancy, consistent with the changes of MAOA protein expression. The summit of MAOB mRNA and protein level was observed on day 4. Immunohistochemistry analysis revealed that both of MAOA and MAOB were mainly localized in the glandular and luminal epithelium cells, as well as their intense staining signals observed in the trophoblast cells on day 6 and 8. Conclusions: Both MAOA and MAOB were up-regulated in the uteri during the peri-implantation period, which could play a role in mouse embryo implantation and endometrial receptivity; the temporal and spatial marked increase of MAOs may be traced as a useful marker for mouse endometrial receptivity; the expression mode of mouse MAOs should pave a way for further study. Springer-Verlag, 2010 Embryo implantation nationallicence Endometrial receptivity nationallicence Monoamine oxidase A nationallicence Monoamine oxidase B nationallicence Mouse uteri nationallicence Zhang Dan Institute of Obstetrics and Gynecology Hospital, Fudan University, 413 Zhaozhou Road, 200011, Shanghai, China aut Lei Caixia Institute of Obstetrics and Gynecology Hospital, Fudan University, 413 Zhaozhou Road, 200011, Shanghai, China aut Zhang Wei Institute of Obstetrics and Gynecology Hospital, Fudan University, 413 Zhaozhou Road, 200011, Shanghai, China aut Archives of Gynecology and Obstetrics Springer-Verlag 284/4(2011-10-01), 861-866 0932-0067 284:4<861 2011 284 404 https://doi.org/10.1007/s00404-010-1765-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-010-1765-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Dan Institute of Obstetrics and Gynecology Hospital, Fudan University, 413 Zhaozhou Road, 200011, Shanghai, China aut NATIONALLICENCE 700 1- Lei Caixia Institute of Obstetrics and Gynecology Hospital, Fudan University, 413 Zhaozhou Road, 200011, Shanghai, China aut NATIONALLICENCE 700 1- Zhang Wei Institute of Obstetrics and Gynecology Hospital, Fudan University, 413 Zhaozhou Road, 200011, Shanghai, China aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 284/4(2011-10-01), 861-866 0932-0067 284:4<861 2011 284 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer