caa a22 4500 445310553 CHVBK 20180317142628.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00404-010-1768-7 doi (NATIONALLICENCE)springer-10.1007/s00404-010-1768-7 Endometrial expression of estrogen receptor β and its splice variants in patients with and without endometriosis [Elektronische Daten] [Ingolf Juhasz-Böss, Claudia Fischer, Claus Lattrich, Maciej Skrzypczak, Eduard Malik, Olaf Ortmann, Oliver Treeck] Background: The role of estrogen receptor beta (ERβ) in pathogenesis of endometriosis remains to be elucidated. In this study, we have examined the expression of the four main ERβ transcript isoforms in human endometrial tissue in women with or without endometriosis. Methods: Total RNA was isolated from native endometrial tissue and transcript levels of ERα, β1, β2, β4, β5 were analyzed by means of RT-PCR. We compared the results with regard to menstrual cycle phase as well as to presence or absence of endometriosis. We prospectively harvested the endometrium of ten women without endometriosis (five for each cycle phase) and eight patients with endometriosis (five in the proliferative phase, three in the secretory phase). Results: ERα, β1, β2, and β5 transcripts were detected in both cycle phases. During the proliferative phase, healthy women had a significantly higher ERα/ERβ1-ratio than patients with endometriosis. Irrespective of the cycle phase, ERα-mRNA level was significantly higher than transcript levels of ERβ isoforms. Conclusions: ERα, β1, β2, and β5 are expressed in human endometrium. The individual receptors differed in terms of expression strength but there was no relevant change during the cycle. The decreased ERα/ERβ1-ratio in proliferative endometrium of endometriosis patients suggest that ERβ1 might be involved in the pathogenesis of endometriosis. Further studies should be undertaken to substantiate the role of ERβ in endometrial pathology. Springer-Verlag, 2010 Estrogen receptor nationallicence ER isoforms nationallicence ER beta nationallicence Endometrium nationallicence Endometriosis nationallicence Juhasz-Böss Ingolf Department of Obstetrics and Gynecology, University of Regensburg, Landshuterstr. 53, 93053, Regensburg, Germany aut Fischer Claudia Department of Obstetrics and Gynecology, University of Regensburg, Landshuterstr. 53, 93053, Regensburg, Germany aut Lattrich Claus Department of Obstetrics and Gynecology, University of Regensburg, Landshuterstr. 53, 93053, Regensburg, Germany aut Skrzypczak Maciej Second Department of Gynecology, Medical University of Lublin, Lublin, Poland aut Malik Eduard Department of Obstetrics and Gynecology, Klinikum Oldenburg, Oldenburg, Germany aut Ortmann Olaf Department of Obstetrics and Gynecology, University of Regensburg, Landshuterstr. 53, 93053, Regensburg, Germany aut Treeck Oliver Department of Obstetrics and Gynecology, University of Regensburg, Landshuterstr. 53, 93053, Regensburg, Germany aut Archives of Gynecology and Obstetrics Springer-Verlag 284/4(2011-10-01), 885-891 0932-0067 284:4<885 2011 284 404 https://doi.org/10.1007/s00404-010-1768-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-010-1768-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Juhasz-Böss Ingolf Department of Obstetrics and Gynecology, University of Regensburg, Landshuterstr. 53, 93053, Regensburg, Germany aut NATIONALLICENCE 700 1- Fischer Claudia Department of Obstetrics and Gynecology, University of Regensburg, Landshuterstr. 53, 93053, Regensburg, Germany aut NATIONALLICENCE 700 1- Lattrich Claus Department of Obstetrics and Gynecology, University of Regensburg, Landshuterstr. 53, 93053, Regensburg, Germany aut NATIONALLICENCE 700 1- Skrzypczak Maciej Second Department of Gynecology, Medical University of Lublin, Lublin, Poland aut NATIONALLICENCE 700 1- Malik Eduard Department of Obstetrics and Gynecology, Klinikum Oldenburg, Oldenburg, Germany aut NATIONALLICENCE 700 1- Ortmann Olaf Department of Obstetrics and Gynecology, University of Regensburg, Landshuterstr. 53, 93053, Regensburg, Germany aut NATIONALLICENCE 700 1- Treeck Oliver Department of Obstetrics and Gynecology, University of Regensburg, Landshuterstr. 53, 93053, Regensburg, Germany aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 284/4(2011-10-01), 885-891 0932-0067 284:4<885 2011 284 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer