caa a22 4500 445310561 CHVBK 20180317142628.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00404-010-1734-4 doi (NATIONALLICENCE)springer-10.1007/s00404-010-1734-4 Inhibin-betaA and -betaB subunits in normal and malignant glandular epithelium of uterine cervix and HeLa cervical cancer cell line [Elektronische Daten] [Alexander Burges, Naim Shabani, Ansgar Brüning, Ioannis Mylonas] Introduction: Inhibins, dimeric peptide hormones composed of an alpha-subunit and one of two possible beta-subunits (betaA or betaB), exhibit substantial roles in human reproduction and in endocrine-responsive tumors. However, it is still unclear if normal and cancerous cervical glandular epithelial cells as well as cervical cancer cell lines of glandular origin express the inhibin-betaA and -betaB subunits. Materials and methods: Normal cervical tissue samples and a total of 10 specimens of well-differentiated adenocarcinomas of the human cervix were analyzed for inhibin-betaA and -betaB subunit expression by immunohistochemical analysis. Additionally, the cervical carcinoma cell line HeLa was analyzed by immunofluorescence and RT-PCR analysis for the expression of inhibin subunits. Results: Immunolabeling of normal and malignant glandular epithelium of human cervical tissue revealed a positive staining reaction for the inhibin-betaA and -betaB subunits. Additionally, the cancer cell line HeLa synthesized both inhibin subunits. When compared to the normal cervical glandular epithelium, the expression of the inhibin beta subunits became significantly reduced in cervical adenocarcinoma tissues. Discussion: In conclusion, we demonstrated a strong, though differential expression pattern of inhibin-betaA and -betaB subunits in normal and malignant glandular epithelial cells of the human uterine cervix. Although the physiological role of inhibins is still quite unclear in cervical tissue, the expression of inhibin-beta-subunits might play an important role in cervical cancer carcinogenesis, since they are significantly down-regulated during pathogenesis in cervical adenocarcinomas. Springer-Verlag, 2010 Cervical cancer, adenocarcinoma nationallicence Cervical cancer cell line nationallicence Hela nationallicence Immunohistochemistry, immunofluorescence, RT-PCR, inhibin-betaA nationallicence Inhibin-betaB nationallicence Burges Alexander Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University Munich, Campus Großhadern, Munich, Germany aut Shabani Naim Department of Obstetrics and Gynaecology, Klinikum Neuperlach, Munich, Germany aut Brüning Ansgar Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University Munich, Campus Großhadern, Munich, Germany aut Mylonas Ioannis 1st Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University Munich, Campus Innenstadt, Maistrasse 11, 80337, Munich, Germany aut Archives of Gynecology and Obstetrics Springer-Verlag 284/4(2011-10-01), 981-988 0932-0067 284:4<981 2011 284 404 https://doi.org/10.1007/s00404-010-1734-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-010-1734-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Burges Alexander Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University Munich, Campus Großhadern, Munich, Germany aut NATIONALLICENCE 700 1- Shabani Naim Department of Obstetrics and Gynaecology, Klinikum Neuperlach, Munich, Germany aut NATIONALLICENCE 700 1- Brüning Ansgar Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University Munich, Campus Großhadern, Munich, Germany aut NATIONALLICENCE 700 1- Mylonas Ioannis 1st Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University Munich, Campus Innenstadt, Maistrasse 11, 80337, Munich, Germany aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 284/4(2011-10-01), 981-988 0932-0067 284:4<981 2011 284 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer