caa a22 4500 445310650 CHVBK 20180317142628.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00404-010-1747-z doi (NATIONALLICENCE)springer-10.1007/s00404-010-1747-z Human papillomavirus genotype distribution in cervical samples collected in routine clinical practice at the Nantes University Hospital, France [Elektronische Daten] [Edouard Vaucel, Marianne Coste-Burel, Christian Laboisse, André Dahlab, Patrice Lopes] Objective: The objective was to assess the human papillomavirus (HPV) overall and type-specific prevalence in smears collected during routine clinical practice. Design: HPV genotyping and smears were performed independently between 2000 and 2006 for routine clinical follow-up (primary screening and follow-up) in the University Hospital of Nantes, France. Population: All women with a cytological sample collected no more than 12months before HPV genotyping were included. Methods: PCR was performed with MY09/MY11 primers and genotyping by sequencing PCR product. Main outcome measures: Overall and genotype-specific HPV prevalence were assessed according to cytological diagnosis. Results: A total of 1,255 women were included (mean age 37.5years). The proportion of high-risk (HR) HPV positive samples increased according to cytological diagnosis severity from 8% in normal specimens to 21% in atypical squamous cells of undetermined significance, 49% in low-grade squamous intraepithelial lesion and 75% in high-grade squamous intraepithelial lesion (HSIL) (p<0.001). Among 980 women with normal cytology, the overall HPV prevalence varied according to age from 44% below 20years to about 10% above 35years (p<0.001). The most prevalent HPV genotype in all cytological diagnoses was HPV 16. HPV 53 appeared as the second most common genotype in normal cytological samples (10.9% of HPV positive samples) but its prevalence decreased in HSIL to less than 4%. Conclusion: The proportion of HR HPV positive women increased according to cytological diagnosis severity. HPV 16 appeared as the most commonly encountered genotype even when the diagnosis was normal. Its prevalence increased with diagnosis severity hereby confirming that HPV 16 is more aggressive than other genotypes. The Author(s), 2010 Human papillomavirus nationallicence Genotype nationallicence Smears nationallicence Normal cytological diagnosis nationallicence ASCUS : Atypical squamous cells of undetermined significance nationallicence CIN : Cervical intraepithelial neoplasia nationallicence HPV : Human papillomavirus nationallicence HSIL : High-grade squamous intraepithelial lesion nationallicence ICC : Invasive squamous cell carcinoma nationallicence LSIL : Low-grade squamous intraepithelial lesion nationallicence Vaucel Edouard CHU de Nantes, Pôle Mère-Enfant, Service de Gynécologie Obstétrique et Médecine de la Reproduction, 7 quai Moncousu, 44093, Nantes Cedex 1, France aut Coste-Burel Marianne CHU de Nantes, Pôle Biologie, IFR 26, Service de Virologie, EA 4271, 44093, Nantes Cedex 1, France aut Laboisse Christian CHU de Nantes, Service d'Anatomie et Cytologie Pathologiques, 44093, Nantes Cedex 1, France aut Dahlab André Sanofi Pasteur MSD, Lyon, France aut Lopes Patrice CHU de Nantes, Pôle Mère-Enfant, Service de Gynécologie Obstétrique et Médecine de la Reproduction, 7 quai Moncousu, 44093, Nantes Cedex 1, France aut Archives of Gynecology and Obstetrics Springer-Verlag 284/4(2011-10-01), 989-998 0932-0067 284:4<989 2011 284 404 https://doi.org/10.1007/s00404-010-1747-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-010-1747-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vaucel Edouard CHU de Nantes, Pôle Mère-Enfant, Service de Gynécologie Obstétrique et Médecine de la Reproduction, 7 quai Moncousu, 44093, Nantes Cedex 1, France aut NATIONALLICENCE 700 1- Coste-Burel Marianne CHU de Nantes, Pôle Biologie, IFR 26, Service de Virologie, EA 4271, 44093, Nantes Cedex 1, France aut NATIONALLICENCE 700 1- Laboisse Christian CHU de Nantes, Service d'Anatomie et Cytologie Pathologiques, 44093, Nantes Cedex 1, France aut NATIONALLICENCE 700 1- Dahlab André Sanofi Pasteur MSD, Lyon, France aut NATIONALLICENCE 700 1- Lopes Patrice CHU de Nantes, Pôle Mère-Enfant, Service de Gynécologie Obstétrique et Médecine de la Reproduction, 7 quai Moncousu, 44093, Nantes Cedex 1, France aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 284/4(2011-10-01), 989-998 0932-0067 284:4<989 2011 284 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer