caa a22 4500 445311053 CHVBK 20180317142630.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00404-010-1782-9 doi (NATIONALLICENCE)springer-10.1007/s00404-010-1782-9 Involvement of ARLTS1 in chemotherapy and apoptosis in ovarian cancer cell line [Elektronische Daten] [Xiao Yang, Hai Yu, Zhi Peng] Purpose: Recent evidence suggests that ADP-ribosylation factor-like tumor suppressor gene 1(ARLTS1) may act as a tumor suppressor gene. However, its role in tumor chemotherapy remains unclear. The aim of this study is to investigate the effects of ARLTS1 gene in regulation of chemosensitivity in ovarian cystadenocarcinoma cell line SKOV3. Methods: We stably expressed wild-type (wt) ARLTS1 and empty vector (neo) in SKOV3 cells. Chemosensitivity test was carried out with four chemotherapeutic agents. Cell proliferation, cycle kinetics and apoptosis were evaluated by MTT assay and flow cytometry. Apoptosis-related proteins caspase-3 and bcl-2 were determined by Western blot analysis. Results: The proliferation of wtARLTS1 clones was more dramatically inhibited by all the cytotoxic agents than parental cells (P<0.05). Increased sensitivity to chemotherapy by two to threefolds was detected in wtARLTS1 cells. The rate of apoptosis in wtARLTS1 was 60.2% treated with DDP (10× peak plasma concentration, PPC), which was dramatically higher than that of neo and parental cells (P is 0.017 and 0.020, respectively). Expression of caspase-3 and bcl-2 in parental cells declined modestly when treated with DDP, while in wtARLTS1 clones the expression of caspase-3 and bcl-2 levels declined more dramatically and become undetectable at lower DDP doses (P=0.023 and <0.001, respectively). Conclusion: Our findings suggested that ARLTS1 may facilitate chemosensitivity in ovarian cancer cells by acting synergistic with chemotherapeutic agents to induce the apoptosis signaling pathway and regulate apoptosis-related proteins. Springer-Verlag, 2010 ARLTS1 nationallicence Ovarian cancer nationallicence Apoptosis nationallicence Chemosensitivity nationallicence Yang Xiao Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China aut Yu Hai Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan, China aut Peng Zhi Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China aut Archives of Gynecology and Obstetrics Springer-Verlag 284/5(2011-11-01), 1241-1246 0932-0067 284:5<1241 2011 284 404 https://doi.org/10.1007/s00404-010-1782-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-010-1782-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Xiao Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Yu Hai Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Peng Zhi Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 284/5(2011-11-01), 1241-1246 0932-0067 284:5<1241 2011 284 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer