caa a22 4500 445311371 CHVBK 20180317142631.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00404-010-1661-4 doi (NATIONALLICENCE)springer-10.1007/s00404-010-1661-4 The effects of different lignans and isoflavones, tested as aglycones and glycosides, on hormone receptor-positive and -negative breast carcinoma cells in vitro [Elektronische Daten] [Conrad Theil, Volker Briese, Bernd Gerber, Dagmar-Ulrike Richter] Purpose: Phytooestrogens are known to cause anti-cancer effects on mamma carcinoma cells. In this study, the effects of the lignan secoisolariciresinol and the isoflavone glycosides and aglycones genistein, genistin, daidzein and daidzin were tested on MCF-7 and BT20 cells in vitro. Methods: First, the cellular expression of hormone receptors was examined by immunohistochemical procedures. The effects of the phytooestrogens on the cells were detected by using three different assays measuring cell letality, viability and proliferation. The phytooestrogens were tested in concentrations of 1, 5, 10 and 50μg/mL, respectively. 17β-oestradiol and tamoxifen were used as controls, respectively, in the same concentrations as the phytooestrogens. Results: The immunohistochemistry showed evidence of oestrogen- and progesterone receptors at the MCF-7 cell line, whereas no expression could be seen at the BT20 cells. Among the phytooestrogens, genistein and secoisolariciresinol showed various anti-cancerogenic effects on both cell lines, respectively, but only in the highest concentration. Regarding the controls, tamoxifen showed a stronger antivital and anti-proliferative effect on BT20 than on MCF-7. Oestradiol caused sporadic anti-cancer effects on both cell lines, respectively, at its highest concentration. Conclusions: Genistein and Secoisolariciresinol have anti-cancer properties on MCF-7 and BT20 in vitro. There are differences in the effects of isoflavones depending on the glycolysation status. The role of the oestrogen receptors in the mechanisms of action of both the phytooestrogens and controls is of less importance. Further investigations have to be carried out, especially concerning the mechanisms of action. Phytooestrogens may be potential substances in the therapy of mamma carcinomas. Springer-Verlag, 2010 Phytooestrogens nationallicence Isoflavones nationallicence Lignans nationallicence Secoisolariciresinol nationallicence Genistein nationallicence Genistin nationallicence Daidzein nationallicence Daidzin nationallicence Breast cancer nationallicence MCF-7 nationallicence BT20 nationallicence Theil Conrad Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Rostock, Südring 81, 18059, Rostock, Germany aut Briese Volker Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Rostock, Südring 81, 18059, Rostock, Germany aut Gerber Bernd Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Rostock, Südring 81, 18059, Rostock, Germany aut Richter Dagmar-Ulrike Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Rostock, Südring 81, 18059, Rostock, Germany aut Archives of Gynecology and Obstetrics Springer-Verlag 284/2(2011-08-01), 459-465 0932-0067 284:2<459 2011 284 404 https://doi.org/10.1007/s00404-010-1661-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-010-1661-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Theil Conrad Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Rostock, Südring 81, 18059, Rostock, Germany aut NATIONALLICENCE 700 1- Briese Volker Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Rostock, Südring 81, 18059, Rostock, Germany aut NATIONALLICENCE 700 1- Gerber Bernd Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Rostock, Südring 81, 18059, Rostock, Germany aut NATIONALLICENCE 700 1- Richter Dagmar-Ulrike Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Rostock, Südring 81, 18059, Rostock, Germany aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 284/2(2011-08-01), 459-465 0932-0067 284:2<459 2011 284 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer