caa a22 4500 44531155X CHVBK 20180317142631.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s11095-010-0169-8 doi (NATIONALLICENCE)springer-10.1007/s11095-010-0169-8 Design, Optimization and Characterisation of Polymeric Microneedle Arrays Prepared by a Novel Laser-Based Micromoulding Technique [Elektronische Daten] [Ryan Donnelly, Rita Majithiya, Thakur Singh, Desmond Morrow, Martin Garland, Yusuf Demir, Katarzyna Migalska, Elizabeth Ryan, David Gillen, Christopher Scott, A. Woolfson] ABSTRACT: Purpose: Design and evaluation of a novel laser-based method for micromoulding of microneedle arrays from polymeric materials under ambient conditions. The aim of this study was to optimise polymeric composition and assess the performance of microneedle devices that possess different geometries. Methods: A range of microneedle geometries was engineered into silicone micromoulds, and their physicochemical features were subsequently characterised. Results: Microneedles micromoulded from 20% w/w aqueous blends of the mucoadhesive copolymer Gantrez® AN-139 were surprisingly found to possess superior physical strength than those produced from commonly used pharma polymers. Gantrez® AN-139 microneedles, 600μm and 900μm in height, penetrated neonatal porcine skin with low application forces (>0.03N per microneedle). When theophylline was loaded into 600μm microneedles, 83% of the incorporated drug was delivered across neonatal porcine skin over 24h. Optical coherence tomography (OCT) showed that drug-free 600μm Gantrez® AN-139 microneedles punctured the stratum corneum barrier of human skin in vivo and extended approximately 460µm into the skin. However, the entirety of the microneedle lengths was not inserted. Conclusion: In this study, we have shown that a novel laser engineering method can be used in micromoulding of polymeric microneedle arrays. We are currently carrying out an extensive OCT-informed study investigating the influence of microneedle array geometry on skin penetration depth, with a view to enhanced transdermal drug delivery from optimised laser-engineered Gantrez® AN-139 microneedles. Springer Science+Business Media, LLC, 2010 laser engineering nationallicence microneedles nationallicence optical coherence tomography nationallicence transdermal drug delivery nationallicence Donnelly Ryan School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut Majithiya Rita School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut Singh Thakur School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut Morrow Desmond School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut Garland Martin School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut Demir Yusuf School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut Migalska Katarzyna School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut Ryan Elizabeth School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut Gillen David Blue Acre Technology, Aiken Business Park, Old Coe's Road, Dundalk, County Louth, Ireland aut Scott Christopher School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut Woolfson A. School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/1(2011-01-01), 41-57 0724-8741 28:1<41 2011 28 11095 https://doi.org/10.1007/s11095-010-0169-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-010-0169-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Donnelly Ryan School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut NATIONALLICENCE 700 1- Majithiya Rita School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut NATIONALLICENCE 700 1- Singh Thakur School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut NATIONALLICENCE 700 1- Morrow Desmond School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut NATIONALLICENCE 700 1- Garland Martin School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut NATIONALLICENCE 700 1- Demir Yusuf School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut NATIONALLICENCE 700 1- Migalska Katarzyna School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut NATIONALLICENCE 700 1- Ryan Elizabeth School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut NATIONALLICENCE 700 1- Gillen David Blue Acre Technology, Aiken Business Park, Old Coe's Road, Dundalk, County Louth, Ireland aut NATIONALLICENCE 700 1- Scott Christopher School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut NATIONALLICENCE 700 1- Woolfson A. School of Pharmacy, Queen's University Belfast Medical Biology Centre, 97 Lisburn Road, BT9 7BL, Belfast, UK aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/1(2011-01-01), 41-57 0724-8741 28:1<41 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer