caa a22 4500 445311606 CHVBK 20180317142632.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s11095-010-0134-6 doi (NATIONALLICENCE)springer-10.1007/s11095-010-0134-6 Stability Kinetics of Influenza Vaccine Coated onto Microneedles During Drying and Storage [Elektronische Daten] [Yeu-Chun Kim, Fu-Shi Quan, Richard Compans, Sang-Moo Kang, Mark Prausnitz] ABSTRACT: Purpose: This study sought to determine the effects of microneedle coating formulation, drying time and storage time on antigen stability and in vivo immunogenicity of influenza microneedle vaccines. Methods: The stability of inactivated influenza virus vaccine was monitored by hemagglutination (HA) activity and virus particle aggregation as a function of storage time and temperature with or without trehalose. In vivo immunogenicity of inactivated influenza vaccines coated onto microneedles was determined in mice by virus-specific antibody titers and survival rates. Results: In the absence of trehalose, HA activity decreased below 10% and to almost zero after 1h and 1month of drying, respectively. Addition of trehalose maintained HA activity above 60% after drying and above 20% after 1month storage at 25°C. Loss of HA activity generally correlated with increased virus particle aggregation. Administration of microneedles coated with trehalose-stabilized influenza vaccine yielded high serum IgG antibody titers even after 1month storage, and all animals survived with minimal weight loss after lethal challenge infection. Conclusions: Inactivated influenza virus vaccine coated on microneedles with trehalose significantly improved the HA activity as well as in vivo immunogenicity of the vaccine after an extended time of storage. Springer Science+Business Media, LLC, 2010 inactivated virus vaccine nationallicence influenza virus nationallicence long-term stability nationallicence microneedle nationallicence trehalose nationallicence Kim Yeu-Chun School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 30332, Atlanta, Georgia, USA aut Quan Fu-Shi Department of Microbiology and Immunology, Emory University School of Medicine, 30322, Atlanta, Georgia, USA aut Compans Richard Department of Microbiology and Immunology, Emory University School of Medicine, 30322, Atlanta, Georgia, USA aut Kang Sang-Moo Department of Microbiology and Immunology, Emory University School of Medicine, 30322, Atlanta, Georgia, USA aut Prausnitz Mark School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 30332, Atlanta, Georgia, USA aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/1(2011-01-01), 135-144 0724-8741 28:1<135 2011 28 11095 https://doi.org/10.1007/s11095-010-0134-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-010-0134-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Yeu-Chun School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 30332, Atlanta, Georgia, USA aut NATIONALLICENCE 700 1- Quan Fu-Shi Department of Microbiology and Immunology, Emory University School of Medicine, 30322, Atlanta, Georgia, USA aut NATIONALLICENCE 700 1- Compans Richard Department of Microbiology and Immunology, Emory University School of Medicine, 30322, Atlanta, Georgia, USA aut NATIONALLICENCE 700 1- Kang Sang-Moo Department of Microbiology and Immunology, Emory University School of Medicine, 30322, Atlanta, Georgia, USA aut NATIONALLICENCE 700 1- Prausnitz Mark School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 30332, Atlanta, Georgia, USA aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/1(2011-01-01), 135-144 0724-8741 28:1<135 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer