caa a22 4500 445311681 CHVBK 20180317142632.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s11095-010-0271-y doi (NATIONALLICENCE)springer-10.1007/s11095-010-0271-y Suprachoroidal Drug Delivery to the Back of the Eye Using Hollow Microneedles [Elektronische Daten] [Samirkumar Patel, Angela Lin, Henry Edelhauser, Mark Prausnitz] ABSTRACT: Purpose: In this work, we tested the hypothesis that microneedles provide a minimally invasive method to inject particles into the suprachoroidal space for drug delivery to the back of the eye. Methods: A single, hollow microneedle was inserted into the sclera, and infused nanoparticle and microparticle suspensions into the suprachoroidal space. Experiments were performed on whole rabbit, pig, and human eyes ex vivo. Particle delivery was imaged using brightfield and fluorescence microscopy as well as microcomputed tomography. Results: Microneedles were shown to deliver sulforhodamine B as well as nanoparticle and microparticle suspensions into the suprachoroidal space of rabbit, pig, and human eyes. Volumes up to 35μL were administered consistently. Optimization of the delivery device parameters showed that microneedle length, pressure, and particle size played an important role in determining successful delivery into the suprachoroidal space. Needle lengths of 800-1,000μm and applied pressures of 250-300kPa provided most reliable delivery. Conclusions: Microneedles were shown for the first time to deliver nanoparticle and microparticle suspensions into the suprachoroidal space of rabbit, pig and human eyes. This shows that microneedles may provide a minimally invasive method for controlled drug delivery to the back of the eye. Springer Science+Business Media, LLC, 2010 eye suprachoroidal space nationallicence hollow microneedle nationallicence microparticle nationallicence nanoparticle nationallicence ocular drug delivery nationallicence Patel Samirkumar School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, 30332-0100, Atlanta, Georgia, USA aut Lin Angela Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 30332, Atlanta, Georgia, USA aut Edelhauser Henry Emory Eye Center, Emory University, 30322, Atlanta, Georgia, USA aut Prausnitz Mark School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, 30332-0100, Atlanta, Georgia, USA aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/1(2011-01-01), 166-176 0724-8741 28:1<166 2011 28 11095 https://doi.org/10.1007/s11095-010-0271-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-010-0271-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Patel Samirkumar School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, 30332-0100, Atlanta, Georgia, USA aut NATIONALLICENCE 700 1- Lin Angela Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 30332, Atlanta, Georgia, USA aut NATIONALLICENCE 700 1- Edelhauser Henry Emory Eye Center, Emory University, 30322, Atlanta, Georgia, USA aut NATIONALLICENCE 700 1- Prausnitz Mark School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, 30332-0100, Atlanta, Georgia, USA aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/1(2011-01-01), 166-176 0724-8741 28:1<166 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer