caa a22 4500 44531169X CHVBK 20180317142632.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s11095-010-0321-5 doi (NATIONALLICENCE)springer-10.1007/s11095-010-0321-5 A Novel Shell-Structure Cell Microcarrier (SSCM) for Cell Transplantation and Bone Regeneration Medicine [Elektronische Daten] [Kai Su, Yihong Gong, Chunming Wang, Dong-An Wang] ABSTRACT: Purpose: The present study aims to develop a novel open and hollow shell-structure cell microcarrier (SSCM) to improve the anchorage-dependent cell (ADC) loading efficiency, increase the space for cell proliferation and tissue regeneration, and better propel its therapeutic effects. Methods: Gelatin particles were prepared with oil/water/oil (o/w/o) technique and modified by an adjustable surface crosslinking technique and subsequent release of uncrosslinked material. Optical microscopy and scanning electron microscopy (SEM) were utilized to observe the morphologies of the microcarriers. Cell loading tests were performed to evaluate the biocompatibilities and effect on osteogenesis of SSCM. Results: SSCMs were successfully fabricated via the surface technique. The shell-structure could allow the cell to attach and grow on both outer and inner surface of sphere and provide adequate space for cell proliferation and extracellular matrix (ECM) secretion. The cell loading rate, proliferation rate and osteogenesis-related gene expressions on the SSCMs were higher than those on the spherical gelatin microcarriers. Conclusions: The outstanding performance of injectable SSCMs endowed with favorable micro-structure, desirable cytocompatibility and enhanced cell affinity makes them as a good choice as cell delivery vehicle for transplanting therapeutic cells towards the scope of tissue regeneration. Springer Science+Business Media, LLC, 2010 cell therapy nationallicence gelatin nationallicence microcarrier nationallicence regenerative medicine nationallicence Su Kai Division of Bioengineering School of Chemical and Biomedical Engineering, Nanyang Technological University, 637457, Singapore, Republic of Singapore aut Gong Yihong Department of Biomedical Engineering, School of Engineering, Sun Yat-sen University, 510275, Guangzhou, China aut Wang Chunming Division of Bioengineering School of Chemical and Biomedical Engineering, Nanyang Technological University, 637457, Singapore, Republic of Singapore aut Wang Dong-An Division of Bioengineering School of Chemical and Biomedical Engineering, Nanyang Technological University, 637457, Singapore, Republic of Singapore aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/6(2011-06-01), 1431-1441 0724-8741 28:6<1431 2011 28 11095 https://doi.org/10.1007/s11095-010-0321-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-010-0321-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Su Kai Division of Bioengineering School of Chemical and Biomedical Engineering, Nanyang Technological University, 637457, Singapore, Republic of Singapore aut NATIONALLICENCE 700 1- Gong Yihong Department of Biomedical Engineering, School of Engineering, Sun Yat-sen University, 510275, Guangzhou, China aut NATIONALLICENCE 700 1- Wang Chunming Division of Bioengineering School of Chemical and Biomedical Engineering, Nanyang Technological University, 637457, Singapore, Republic of Singapore aut NATIONALLICENCE 700 1- Wang Dong-An Division of Bioengineering School of Chemical and Biomedical Engineering, Nanyang Technological University, 637457, Singapore, Republic of Singapore aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/6(2011-06-01), 1431-1441 0724-8741 28:6<1431 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer