caa a22 4500 445311711 CHVBK 20180317142632.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s11095-010-0352-y doi (NATIONALLICENCE)springer-10.1007/s11095-010-0352-y The Effect of Human Bone Marrow Stroma-Derived Heparan Sulfate on the Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells [Elektronische Daten] [Diah Bramono, David Rider, Sadasivam Murali, Victor Nurcombe, Simon Cool] ABSTRACT: Purpose: In order to address cell dose limitations associated with the use of cord blood hematopoietic stem cell (HSC) transplantation, we explored the effect of bone marrow stroma-derived heparan sulfate (HS) on the ex vivo expansion of HSCs. Methods: Heparan sulfate was isolated and purified from the conditioned media of human bone marrow stromal cells and used for the expansion of cord blood-derived CD34+ cells in the presence of a cocktail of cytokines. Results: The number of myeloid lineage-committed progenitor cells was increased at low dosage of HS as illustrated by an increase in the total number of colony-forming cells (CFC) and colonies of erythroid (BFU-E) and granulocyte-macrophage (CFU-GM) precursors. Notably, the stroma-derived HS did not alter the growth of CD34+ HSCs or negatively affect the levels of various HSC phenotypic markers after expansion. Conclusions: This study shows that HS secreted into solution by stromal cells has the capacity to support hematopoietic cytokines in the maintenance and expansion of HSCs. The incorporation of stroma-derived HS as a reagent may improve the efficacy of cord blood HSC transplantation by enhancing the number of committed cells and accelerating the rate of engraftment. Springer Science+Business Media, LLC, 2010 cord blood nationallicence glycosaminoglycan nationallicence hematopoietic stem cell nationallicence heparan sulfate nationallicence stem cell expansion nationallicence Bramono Diah Stem Cells and Tissue Repair Group, Institute of Medical Biology, A*STAR (Agency for Science, Technology and Research), 8A Biomedical Grove, #06-06 Immunos, 138648, Singapore, Singapore aut Rider David Stem Cells and Tissue Repair Group, Institute of Medical Biology, A*STAR (Agency for Science, Technology and Research), 8A Biomedical Grove, #06-06 Immunos, 138648, Singapore, Singapore aut Murali Sadasivam Stem Cells and Tissue Repair Group, Institute of Medical Biology, A*STAR (Agency for Science, Technology and Research), 8A Biomedical Grove, #06-06 Immunos, 138648, Singapore, Singapore aut Nurcombe Victor Stem Cells and Tissue Repair Group, Institute of Medical Biology, A*STAR (Agency for Science, Technology and Research), 8A Biomedical Grove, #06-06 Immunos, 138648, Singapore, Singapore aut Cool Simon Stem Cells and Tissue Repair Group, Institute of Medical Biology, A*STAR (Agency for Science, Technology and Research), 8A Biomedical Grove, #06-06 Immunos, 138648, Singapore, Singapore aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/6(2011-06-01), 1385-1394 0724-8741 28:6<1385 2011 28 11095 https://doi.org/10.1007/s11095-010-0352-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-010-0352-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bramono Diah Stem Cells and Tissue Repair Group, Institute of Medical Biology, A*STAR (Agency for Science, Technology and Research), 8A Biomedical Grove, #06-06 Immunos, 138648, Singapore, Singapore aut NATIONALLICENCE 700 1- Rider David Stem Cells and Tissue Repair Group, Institute of Medical Biology, A*STAR (Agency for Science, Technology and Research), 8A Biomedical Grove, #06-06 Immunos, 138648, Singapore, Singapore aut NATIONALLICENCE 700 1- Murali Sadasivam Stem Cells and Tissue Repair Group, Institute of Medical Biology, A*STAR (Agency for Science, Technology and Research), 8A Biomedical Grove, #06-06 Immunos, 138648, Singapore, Singapore aut NATIONALLICENCE 700 1- Nurcombe Victor Stem Cells and Tissue Repair Group, Institute of Medical Biology, A*STAR (Agency for Science, Technology and Research), 8A Biomedical Grove, #06-06 Immunos, 138648, Singapore, Singapore aut NATIONALLICENCE 700 1- Cool Simon Stem Cells and Tissue Repair Group, Institute of Medical Biology, A*STAR (Agency for Science, Technology and Research), 8A Biomedical Grove, #06-06 Immunos, 138648, Singapore, Singapore aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/6(2011-06-01), 1385-1394 0724-8741 28:6<1385 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer