caa a22 4500 44531172X CHVBK 20180317142632.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s11095-010-0320-6 doi (NATIONALLICENCE)springer-10.1007/s11095-010-0320-6 Bioactive Electrospun Scaffolds Delivering Growth Factors and Genes for Tissue Engineering Applications [Elektronische Daten] [Wei Ji, Yan Sun, Fang Yang, Jeroen van den Beucken, Mingwen Fan, Zhi Chen, John Jansen] ABSTRACT: A biomaterial scaffold is one of the key factors for successful tissue engineering. In recent years, an increasing tendency has been observed toward the combination of scaffolds and biomolecules, e.g. growth factors and therapeutic genes, to achieve bioactive scaffolds, which not only provide physical support but also express biological signals to modulate tissue regeneration. Huge efforts have been made on the exploration of strategies to prepare bioactive scaffolds. Within the past fiveyears, electrospun scaffolds have gained an exponentially increasing popularity in this area because of their ultrathin fiber diameter and large surface-volume ratio, which is favored for biomolecule delivery. This paper reviews current techniques that can be used to prepare bioactive electrospun scaffolds, including physical adsorption, blend electrospinning, coaxial electrospinning, and covalent immobilization. In addition, this paper also analyzes the existing challenges (i.e., protein instability, low gene transfection efficiency, and difficulties in accurate kinetics prediction) to achieve biomolecule release from electrospun scaffolds, which necessitate further research to fully exploit the biomedical applications of these bioactive scaffolds. The Author(s), 2010 electrospinning nationallicence gene delivery nationallicence protein delivery nationallicence scaffold nationallicence tissue engineering nationallicence ALP : alkaline phosphatase nationallicence BMP2 : bone morphogenic protein 2 (protein form) nationallicence bmp2 : bone morphogenic protein 2 (gene form) nationallicence BSA : bovine serum albumin nationallicence EGF : epidermal growth factor nationallicence FA : folic acid nationallicence HA : hyaluronic acid nationallicence HAp : hydroxylapatite nationallicence NGF : nerve growth factor nationallicence pBMP-2 : plasmid DNA encoding bone morphogenic protein-2 nationallicence PCL : poly(ε-caprolactone) nationallicence PCL-b-PEG : poly(ε-caprolactone)-block-poly(ethylene glycol) nationallicence pCMV-EGFP : plasmid DNA encoding enhanced green fluorescent protein with a cytomegalovirus promoter nationallicence pCMVβ : plasmid DNA encoding β-galactosidase nationallicence PDGF-bb : platelet-derived growth factor-bb nationallicence PDLLA : poly (D,L-lactide) nationallicence pDNA : plasmid deoxyribonucleic acid nationallicence PEG-b-PDLLA : poly (ethylene glycol)-block-poly(D,L-lactide) nationallicence pEGFP-N1 : plasmid DNA encoding a red shifted variant of wild-type green fluorescent protein nationallicence pGL3 : plasmid DNA encoding luciferase nationallicence PLCL : poly(L-lactide-co-epsilon-caprolactone) nationallicence PLGA : poly(lactide-co-glycolide) nationallicence PMMAAA : copolymer of methyl methacrylate (MMA) and acrylic acid (AA) nationallicence PSU : polysulphone nationallicence PVA : poly(vinyl alcohol) nationallicence Ji Wei Department of Biomaterials (Dentistry 309), Radboud University Nijmegen Medical Center, PO Box9101, 6500 HB, Nijmegen, The Netherlands aut Sun Yan Department of Biomaterials (Dentistry 309), Radboud University Nijmegen Medical Center, PO Box9101, 6500 HB, Nijmegen, The Netherlands aut Yang Fang Department of Biomaterials (Dentistry 309), Radboud University Nijmegen Medical Center, PO Box9101, 6500 HB, Nijmegen, The Netherlands aut van den Beucken Jeroen Department of Biomaterials (Dentistry 309), Radboud University Nijmegen Medical Center, PO Box9101, 6500 HB, Nijmegen, The Netherlands aut Fan Mingwen Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, 430079, Wuhan, Hubei Province, People's Republic of China aut Chen Zhi Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, 430079, Wuhan, Hubei Province, People's Republic of China aut Jansen John Department of Biomaterials (Dentistry 309), Radboud University Nijmegen Medical Center, PO Box9101, 6500 HB, Nijmegen, The Netherlands aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/6(2011-06-01), 1259-1272 0724-8741 28:6<1259 2011 28 11095 https://doi.org/10.1007/s11095-010-0320-6 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-010-0320-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ji Wei Department of Biomaterials (Dentistry 309), Radboud University Nijmegen Medical Center, PO Box9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Sun Yan Department of Biomaterials (Dentistry 309), Radboud University Nijmegen Medical Center, PO Box9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Yang Fang Department of Biomaterials (Dentistry 309), Radboud University Nijmegen Medical Center, PO Box9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- van den Beucken Jeroen Department of Biomaterials (Dentistry 309), Radboud University Nijmegen Medical Center, PO Box9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Fan Mingwen Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, 430079, Wuhan, Hubei Province, People's Republic of China aut NATIONALLICENCE 700 1- Chen Zhi Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, 430079, Wuhan, Hubei Province, People's Republic of China aut NATIONALLICENCE 700 1- Jansen John Department of Biomaterials (Dentistry 309), Radboud University Nijmegen Medical Center, PO Box9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/6(2011-06-01), 1259-1272 0724-8741 28:6<1259 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer