caa a22 4500 445311754 CHVBK 20180317142632.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s11095-011-0452-3 doi (NATIONALLICENCE)springer-10.1007/s11095-011-0452-3 Combination of Hyaluronic Acid Hydrogel Scaffold and PLGA Microspheres for Supporting Survival of Neural Stem Cells [Elektronische Daten] [Ying Wang, Yue Wei, Zhao Zu, Rong Ju, Mu Guo, Xiu Wang, Qun Xu, Fu Cui] ABSTRACT: Purpose: To develop a biomaterial composite for promoting proliferation and migration of neural stem cells (NSCs), as well as angiogenesis on the materials, to rescue central nervous system (CNS) injuries. Methods: A delivery system was constructed based on cross-linked hyaluronic acid (HA) hydrogels, containing embedded BDNF and VEGF-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres for controlled delivery and support for NSCs in the CNS. The surface morphologies were evaluated by SEM and AFM, mechanical property was investigated by rheological tests, and release kinetics were performed by ELISA. Bioactivity of released BDNF and VEGF was assessed by neuron and endothelial cell culture, respectively. Compatibility with NSCs was studied by immunofluorescent staining. Results: Release kinetics showed the delivery of BDNF and VEGF from PLGA microspheres and HA hydrogel composite were sustainable and stable, releasing ~20-30% within 150h. The bioactivities preserved well to promote survival and growth of the cells. Evaluation of structure and mechanical properties showed the hydrogel composite possessed an elastic scaffold structure. Biocompatibility assay showed NSCs adhered and proliferated well on the hydrogel. Conclusions: Our created HA hydrogel/PLGA microsphere systems have a good potential for controlled delivery of varied biofactors and supporting NSCs for brain repair and implantation. Springer Science+Business Media, LLC, 2011 central nervous system nationallicence controlled release nationallicence growth factor nationallicence hyaluronic acid hydrogel nationallicence PLGA microsphere nationallicence BDNF : brain-derived neurotrophic factor nationallicence CNS : central nervous system nationallicence ECM : extracellular matrix nationallicence HA : Hyaluronic acid nationallicence NSC : neural stem cell nationallicence PLGA : poly(lactic-co-glycolic acid) nationallicence VEGF : vascular endothelial growth factor nationallicence Wang Ying Beijing Institute for Neuroscience, Beijing Center of Neutral Regeneration and Repair, Key Laboratory for Neurodegenerative Disease of The Ministry of Education, Capital Medical University, 100069, Beijing, China aut Wei Yue Institute of Regenerative Medical and Biomimetic Materials Department of Materials Science and Engineering,, Tsinghua University, 100084, Beijing, China aut Zu Zhao Department of Neurosurgery FuXing Hospital,, Capital Medical University, 100038, Beijing, China aut Ju Rong Beijing Institute for Neuroscience, Beijing Center of Neutral Regeneration and Repair, Key Laboratory for Neurodegenerative Disease of The Ministry of Education, Capital Medical University, 100069, Beijing, China aut Guo Mu Institute of Regenerative Medical and Biomimetic Materials Department of Materials Science and Engineering,, Tsinghua University, 100084, Beijing, China aut Wang Xiu Institute of Regenerative Medical and Biomimetic Materials Department of Materials Science and Engineering,, Tsinghua University, 100084, Beijing, China aut Xu Qun Beijing Institute for Neuroscience, Beijing Center of Neutral Regeneration and Repair, Key Laboratory for Neurodegenerative Disease of The Ministry of Education, Capital Medical University, 100069, Beijing, China aut Cui Fu Institute of Regenerative Medical and Biomimetic Materials Department of Materials Science and Engineering,, Tsinghua University, 100084, Beijing, China aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/6(2011-06-01), 1406-1414 0724-8741 28:6<1406 2011 28 11095 https://doi.org/10.1007/s11095-011-0452-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-011-0452-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Ying Beijing Institute for Neuroscience, Beijing Center of Neutral Regeneration and Repair, Key Laboratory for Neurodegenerative Disease of The Ministry of Education, Capital Medical University, 100069, Beijing, China aut NATIONALLICENCE 700 1- Wei Yue Institute of Regenerative Medical and Biomimetic Materials Department of Materials Science and Engineering,, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Zu Zhao Department of Neurosurgery FuXing Hospital,, Capital Medical University, 100038, Beijing, China aut NATIONALLICENCE 700 1- Ju Rong Beijing Institute for Neuroscience, Beijing Center of Neutral Regeneration and Repair, Key Laboratory for Neurodegenerative Disease of The Ministry of Education, Capital Medical University, 100069, Beijing, China aut NATIONALLICENCE 700 1- Guo Mu Institute of Regenerative Medical and Biomimetic Materials Department of Materials Science and Engineering,, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Wang Xiu Institute of Regenerative Medical and Biomimetic Materials Department of Materials Science and Engineering,, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Xu Qun Beijing Institute for Neuroscience, Beijing Center of Neutral Regeneration and Repair, Key Laboratory for Neurodegenerative Disease of The Ministry of Education, Capital Medical University, 100069, Beijing, China aut NATIONALLICENCE 700 1- Cui Fu Institute of Regenerative Medical and Biomimetic Materials Department of Materials Science and Engineering,, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/6(2011-06-01), 1406-1414 0724-8741 28:6<1406 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer