caa a22 4500 445311762 CHVBK 20180317142632.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s11095-011-0391-z doi (NATIONALLICENCE)springer-10.1007/s11095-011-0391-z Controlled Release of IGF-1 and HGF from a Biodegradable Polyurethane Scaffold [Elektronische Daten] [Devin Nelson, Priya Baraniak, Zuwei Ma, Jianjun Guan, N. Mason, William Wagner] ABSTRACT: Purpose: Biodegradable elastomers, which can possess favorable mechanical properties and degradation rates for soft tissue engineering applications, are more recently being explored as depots for biomolecule delivery. The objective of this study was to synthesize and process biodegradable, elastomeric poly(ester urethane)urea (PEUU) scaffolds and to characterize their ability to incorporate and release bioactive insulin-like growth factor-1 (IGF-1) and hepatocyte growth factor (HGF). Methods: Porous PEUU scaffolds made from either 5 or 8wt% PEUU were prepared with direct growth-factor incorporation. Long-term in vitro IGF-1 release kinetics were investigated in saline or saline with 100 units/ml lipase to simulate in vivo degradation. Cellular assays were used to confirm released IGF-1 and HGF bioactivity. Results: IGF-1 release into saline occurred in a complex multi-phasic manner for up to 440days. Scaffolds generated from 5wt% PEUU delivered protein faster than 8wt% scaffolds. Lipase-accelerated scaffold degradation led to delivery of >90% protein over 9weeks for both polymer concentrations. IGF-1 and HGF bioactivity in the first 3weeks was confirmed. Conclusions: The capacity of a biodegradable elastomeric scaffold to provide long-term growth-factor delivery was demonstrated. Such a system might provide functional benefit in cardiovascular and other soft tissue engineering applications. Springer Science+Business Media, LLC, 2011 elastomer nationallicence growth factor delivery nationallicence polyurethane nationallicence scaffold nationallicence tissue engineering nationallicence BDI : 1,4-diisocyanatobutane nationallicence bFGF : basic fibroblast growth factor nationallicence BSA : bovine serum albumin nationallicence DMEM : Dulbecco's modified Eagle medium nationallicence DMSO : dimethyl sulfoxide nationallicence HGF : hepatocyte growth factor nationallicence HUVECs : human umbilical vein endothelial cells nationallicence IGF-1 : insulin-like growth factor-1 nationallicence MEM : minimum essential medium nationallicence PBS : phosphate-buffered saline nationallicence PCL : polycaprolactone nationallicence PEUU : poly(ester urethane)urea nationallicence TIPS : thermally induced phase separation nationallicence Nelson Devin Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA aut Baraniak Priya Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA aut Ma Zuwei McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA aut Guan Jianjun McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA aut Mason N. Department of Radiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA aut Wagner William Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/6(2011-06-01), 1282-1293 0724-8741 28:6<1282 2011 28 11095 https://doi.org/10.1007/s11095-011-0391-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-011-0391-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nelson Devin Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA aut NATIONALLICENCE 700 1- Baraniak Priya Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA aut NATIONALLICENCE 700 1- Ma Zuwei McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA aut NATIONALLICENCE 700 1- Guan Jianjun McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA aut NATIONALLICENCE 700 1- Mason N. Department of Radiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA aut NATIONALLICENCE 700 1- Wagner William Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/6(2011-06-01), 1282-1293 0724-8741 28:6<1282 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer