caa a22 4500 445311770 CHVBK 20180317142632.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s11095-011-0445-2 doi (NATIONALLICENCE)springer-10.1007/s11095-011-0445-2 Chondrogenic Priming Adipose-Mesenchymal Stem Cells for Cartilage Tissue Regeneration [Elektronische Daten] [Nathaniel Hwang, Sung Im, Patrick Wu, David Bichara, Xing Zhao, Mark Randolph, Robert Langer, Daniel Anderson] ABSTRACT: Purpose: Chondrocytes lose their ability to produce cartilaginous matrix during multiplication in culture through repeated passages, resulting in inferior tissue phenotype. To overcome the limited amount of primary chondrocytes, we aimed to determine the optimal culture condition for in vitro/in vivo cartilage regeneration using human adipose-derived mesenchymal stem cells (AMSCs). Methods: To evaluate the effects exerted by the chondrocytic culture condition on AMSC, we utilized chondrocyte conditioned medium (CM) and/or co-culture methods to prime and differentiate AMSCs. We evaluated ultimate in vivo engineered cartilage with primed AMSCs with that of chondrocytes. To examine the link between conditioned factors and proliferation/differentiation, cell cycle progression of AMSCs were examined using 5-ethynyl-2′-deoxyuridine (EdU), and gene expression was monitored. Results: We report that AMSCs can be stimulated to become chondrogenic cells when expanded with chondrocyte CM. Polymeric scaffolds co-seeded with CM- expanded AMSCs and primary chondrocytes resulted in in vivo cartilaginous tissues with similar biochemical content to constructs seeded with chondrocytes alone. Conclusion: These results indicate that chondrocyte CM consists of suitable morphogenetic factors that induce the chondrogenic priming of AMSCs for cartilage tissue engineering. Springer Science+Business Media, LLC, 2011 auricular chondrocytes nationallicence cartilage nationallicence co-culture nationallicence human adipose derived mesenchymal stem cells nationallicence tissue engineering nationallicence Hwang Nathaniel David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts, USA aut Im Sung Department of Chemical Engineering, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts, USA aut Wu Patrick David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts, USA aut Bichara David Plastic Surgery Research Laboratory, Massachusetts General Hospital, Harvard Medical School, 02114, Boston, Massachusetts, USA aut Zhao Xing Plastic Surgery Research Laboratory, Massachusetts General Hospital, Harvard Medical School, 02114, Boston, Massachusetts, USA aut Randolph Mark Plastic Surgery Research Laboratory, Massachusetts General Hospital, Harvard Medical School, 02114, Boston, Massachusetts, USA aut Langer Robert David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts, USA aut Anderson Daniel David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts, USA aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/6(2011-06-01), 1395-1405 0724-8741 28:6<1395 2011 28 11095 https://doi.org/10.1007/s11095-011-0445-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-011-0445-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hwang Nathaniel David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts, USA aut NATIONALLICENCE 700 1- Im Sung Department of Chemical Engineering, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts, USA aut NATIONALLICENCE 700 1- Wu Patrick David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts, USA aut NATIONALLICENCE 700 1- Bichara David Plastic Surgery Research Laboratory, Massachusetts General Hospital, Harvard Medical School, 02114, Boston, Massachusetts, USA aut NATIONALLICENCE 700 1- Zhao Xing Plastic Surgery Research Laboratory, Massachusetts General Hospital, Harvard Medical School, 02114, Boston, Massachusetts, USA aut NATIONALLICENCE 700 1- Randolph Mark Plastic Surgery Research Laboratory, Massachusetts General Hospital, Harvard Medical School, 02114, Boston, Massachusetts, USA aut NATIONALLICENCE 700 1- Langer Robert David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts, USA aut NATIONALLICENCE 700 1- Anderson Daniel David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts, USA aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/6(2011-06-01), 1395-1405 0724-8741 28:6<1395 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer