caa a22 4500 445311835 CHVBK 20180317142632.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s11095-011-0378-9 doi (NATIONALLICENCE)springer-10.1007/s11095-011-0378-9 Influence of Physical Properties of Biomaterials on Cellular Behavior [Elektronische Daten] [Susan Lin, Nivedita Sangaj, Tojo Razafiarison, Chao Zhang, Shyni Varghese] ABSTRACT: Purpose: In this study, we evaluated the effect of hydrogel structural properties on proliferation and biosynthesis activity of encapsulated chondrocytes. Methods: Hydrogels with varying structural and mechanical properties were prepared by photopolymerizing PEGDA precursors having MWs of 3.4kDa, 6kDa, 10kDa, and 20kDa and were characterized for their swelling ratio, network structure, morphology, and mechanical properties. The effect of hydrogel structural properties on the cellular activity of encapsulated chondrocytes was studied over four weeks. Results: Varying the molecular weight of PEGDA precursors exhibited a significant effect on the structural and mechanical properties of the hydrogels. Large mesh size was found to support cell proliferation. However, extracellular matrix (ECM) accumulation varied with the precursor molecular weight. Both PEGDA 6kDa and 10kDa hydrogels supported GAG accumulation, while PEGDA 10kDa and 20KDa hydrogels supported collagen accumulation. Chondrocytes cultured in PEGDA 10kDa hydrogels expressed a relative increase in collagen type II and aggrecan expression while maintaining low collagen type I expression. Conclusions: Increasing mesh size of the hydrogels resulted in an increase in cellular proliferation exhibiting the strong correlation between mesh size and cell growth, while mesh size had a differential effect on ECM accumulation and expression of cartilage specific markers. The Author(s), 2011 cartilage nationallicence chondrocytes nationallicence collagen nationallicence GAG nationallicence mesh size nationallicence scaffold nationallicence ECM : extracellular matrix nationallicence GAG : glycosaminoglycan nationallicence PEG : poly(ethylene glycol) nationallicence SR : swelling ratio nationallicence $$ {\overline M_c} $$ : average molecular weight between two adjacent crosslinks nationallicence ξ : mesh size nationallicence Lin Susan Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, 92093-0412, La Jolla, California, USA aut Sangaj Nivedita Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, 92093-0412, La Jolla, California, USA aut Razafiarison Tojo Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, 92093-0412, La Jolla, California, USA aut Zhang Chao Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, 92093-0412, La Jolla, California, USA aut Varghese Shyni Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, 92093-0412, La Jolla, California, USA aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/6(2011-06-01), 1422-1430 0724-8741 28:6<1422 2011 28 11095 https://doi.org/10.1007/s11095-011-0378-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-011-0378-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lin Susan Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, 92093-0412, La Jolla, California, USA aut NATIONALLICENCE 700 1- Sangaj Nivedita Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, 92093-0412, La Jolla, California, USA aut NATIONALLICENCE 700 1- Razafiarison Tojo Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, 92093-0412, La Jolla, California, USA aut NATIONALLICENCE 700 1- Zhang Chao Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, 92093-0412, La Jolla, California, USA aut NATIONALLICENCE 700 1- Varghese Shyni Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, 92093-0412, La Jolla, California, USA aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/6(2011-06-01), 1422-1430 0724-8741 28:6<1422 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer