caa a22 4500 445311878 CHVBK 20180317142633.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s11095-010-0358-5 doi (NATIONALLICENCE)springer-10.1007/s11095-010-0358-5 Porous EH and EH-PEG Scaffolds as Gene Delivery Vehicles to Skeletal Muscle [Elektronische Daten] [Erin Falco, Martha Wang, Joshua Thompson, Joshua Chetta, Diana Yoon, Erik Li, Mangesh Kulkami, Sameer Shah, Abhay Pandit, J. Roth, John Fisher] ABSTRACT: Purpose: Synthetic biomaterials are widely used in an attempt to control the cellular behavior of regenerative tissues. This can be done by altering the chemical and physical properties of the polymeric scaffold to guide tissue repair. This paper addresses the use of a polymeric scaffold (EH network) made from the cyclic acetal monomer, 5-ethyl-5-(hydroxymethyl)-β,β-dimethyl-1,3-dioxane-2-ethanol diacrylate (EHD), as a release device for a therapeutic plasmid encoding for an insulin-like growth factor-1 green fluorescent protein fusion protein (IGF-1 GFP). Methods: Scaffolds were designed to have different porous architectures, and the impact of these architectures on plasmid release was determined. We hypothesized that IGF-1 could be delivered more effectively using a porous scaffold to allow for the release of IGF-1. Results: We showed that by altering the number of pores exposed to the surface of the network, faster plasmid loading and release were achieved. In addition, the IGF-1 GFP plasmids were found to be effective in producing IGF-1 and GFP within human skeletal muscle myoblast cell cultures. Conclusions: This work aims to show the utility of EH biomaterials for plasmid delivery for potentially localized skeletal muscle regeneration. Springer Science+Business Media, LLC, 2011 cyclic acetal nationallicence gene therapy nationallicence IGF-1 nationallicence synthetic meshes nationallicence Falco Erin Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, USA aut Wang Martha Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Building, 20742, College Park, Maryland, USA aut Thompson Joshua Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Building, 20742, College Park, Maryland, USA aut Chetta Joshua Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Building, 20742, College Park, Maryland, USA aut Yoon Diana Department of Bioengineering, Rice University, Houston, Texas, USA aut Li Erik Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Building, 20742, College Park, Maryland, USA aut Kulkami Mangesh National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland aut Shah Sameer Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Building, 20742, College Park, Maryland, USA aut Pandit Abhay National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland aut Roth J. Department of Surgery, University of Kentucky College of Medicine, Lexington, Kentucky, USA aut Fisher John Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Building, 20742, College Park, Maryland, USA aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/6(2011-06-01), 1306-1316 0724-8741 28:6<1306 2011 28 11095 https://doi.org/10.1007/s11095-010-0358-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-010-0358-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Falco Erin Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, USA aut NATIONALLICENCE 700 1- Wang Martha Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Building, 20742, College Park, Maryland, USA aut NATIONALLICENCE 700 1- Thompson Joshua Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Building, 20742, College Park, Maryland, USA aut NATIONALLICENCE 700 1- Chetta Joshua Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Building, 20742, College Park, Maryland, USA aut NATIONALLICENCE 700 1- Yoon Diana Department of Bioengineering, Rice University, Houston, Texas, USA aut NATIONALLICENCE 700 1- Li Erik Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Building, 20742, College Park, Maryland, USA aut NATIONALLICENCE 700 1- Kulkami Mangesh National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland aut NATIONALLICENCE 700 1- Shah Sameer Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Building, 20742, College Park, Maryland, USA aut NATIONALLICENCE 700 1- Pandit Abhay National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland aut NATIONALLICENCE 700 1- Roth J. Department of Surgery, University of Kentucky College of Medicine, Lexington, Kentucky, USA aut NATIONALLICENCE 700 1- Fisher John Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Building, 20742, College Park, Maryland, USA aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/6(2011-06-01), 1306-1316 0724-8741 28:6<1306 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer