caa a22 4500 445311924 CHVBK 20180317142633.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s11095-011-0455-0 doi (NATIONALLICENCE)springer-10.1007/s11095-011-0455-0 Structure-Skin Permeability Relationship of Dendrimers [Elektronische Daten] [Venkata Venuganti, Preety Sahdev, Michael Hildreth, Xiangming Guan, Omathanu Perumal] ABSTRACT: Purpose: To investigate skin penetration of poly (amidoamine) (PAMAM) dendrimers as a function of surface charge and molecular weight in presence and absence of iontophoresis. Methods: Dendrimers were labeled with fluoroisothiocynate (FITC); skin penetration of dendrimers was studied using excised porcine skin in-vitro. Skin penetration of FITC-labeled dendrimers was quantified using confocal laser scanning microscope (CLSM). G2-G6 NH2, G3.5-COOH and G4-OH dendrimers were used. Results: Cationic dendrimers showed higher skin penetration than neutral and anionic dendrimers. Skin penetration of cationic dendrimer increased linearly with increase in treatment time. Iontophoresis enhanced skin penetration of cationic and neutral dendrimers. Increase in current strength and current duration increased skin transport of dendrimers. Passive and iontophoretic skin penetration of cationic dendrimers was inversely related to their molecular weight. Dendrimer penetrated the skin through intercellular lipids and hair follicles. With iontophoresis, dendrimer was also found in localized skin regions. Conclusions: The study demonstrates that the physicochemical properties of dendrimers influence their skin transport. Findings can be used to design dendrimer-based nanocarriers for drug delivery to skin. Springer Science+Business Media, LLC, 2011 confocal laser scanning microscope nationallicence dendrimer charge nationallicence dendrimer generation nationallicence iontophoresis nationallicence nanocarrier nationallicence Venuganti Venkata Pharmacy Division Birla Institute of Technology and Science, Pilani - Hyderabad Campus Jawahar Nagar Shameerpet Mandal, 500078, Hyderabad, Andhra Pradesh, India aut Sahdev Preety Department of Pharmaceutical Sciences, South Dakota State University, 57007, Brookings, South Dakota, USA aut Hildreth Michael Department of Biology and Microbiology, South Dakota State University, 57007, Brookings, South Dakota, USA aut Guan Xiangming Department of Pharmaceutical Sciences, South Dakota State University, 57007, Brookings, South Dakota, USA aut Perumal Omathanu Department of Pharmaceutical Sciences, South Dakota State University, 57007, Brookings, South Dakota, USA aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/9(2011-09-01), 2246-2260 0724-8741 28:9<2246 2011 28 11095 https://doi.org/10.1007/s11095-011-0455-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-011-0455-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Venuganti Venkata Pharmacy Division Birla Institute of Technology and Science, Pilani - Hyderabad Campus Jawahar Nagar Shameerpet Mandal, 500078, Hyderabad, Andhra Pradesh, India aut NATIONALLICENCE 700 1- Sahdev Preety Department of Pharmaceutical Sciences, South Dakota State University, 57007, Brookings, South Dakota, USA aut NATIONALLICENCE 700 1- Hildreth Michael Department of Biology and Microbiology, South Dakota State University, 57007, Brookings, South Dakota, USA aut NATIONALLICENCE 700 1- Guan Xiangming Department of Pharmaceutical Sciences, South Dakota State University, 57007, Brookings, South Dakota, USA aut NATIONALLICENCE 700 1- Perumal Omathanu Department of Pharmaceutical Sciences, South Dakota State University, 57007, Brookings, South Dakota, USA aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/9(2011-09-01), 2246-2260 0724-8741 28:9<2246 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer