caa a22 4500 445311959 CHVBK 20180317142633.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s11095-011-0459-9 doi (NATIONALLICENCE)springer-10.1007/s11095-011-0459-9 Activation of Antigen-Specific T Cell-Responses by Mannan-Decorated PLGA Nanoparticles [Elektronische Daten] [Samar Hamdy, Azita Haddadi, Anooshirvan Shayeganpour, John Samuel, Afsaneh Lavasanifar] ABSTRACT: Purpose: Mannosylation of vaccines is a promising strategy to selectively target vaccine antigens to the mannose receptor expressed on dendritic cells (DCs). The purpose of this study was to investigate the effect of mannan (MN) chemically conjugated to poly(D, L-lactide-co-glycolic acid) (PLGA) nanoparticles (NPs) on antigen-specific T-cell responses elicited by a model antigen (ovalbumin, OVA) loaded in PLGA-NPs. Methods: In vitro T-cell proliferation assay was done to assess the ability of DCs treated with OVA-NPs (±MN decoration) to induce antigen-specific T-cell activation. The efficacy of this vaccination strategy was further evaluated in vivo, where T-cell proliferation was performed to evaluate activation of T-cell responses in lymph nodes and spleens isolated from the vaccinated mice. Results: Our results demonstrate that MN-decorated antigen-loaded PLGA-NPs simultaneously enhanced antigen-specific CD4+ and CD8+ T-cell responses compared to non-decorated NPs. Conclusions: MN decoration of PLGA-NPs is a promising strategy for enhancing antigen-specific T-cell responses. Springer Science+Business Media, LLC, 2011 dendritic cells nationallicence Mannan nationallicence PLGA nationallicence targeting nationallicence vaccine nationallicence Hamdy Samar Faculty of Pharmacy and Pharmaceutical Sciences 3133 Dentistry/Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada aut Haddadi Azita Division of Pharmacy, College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, Saskatchewan, Canada aut Shayeganpour Anooshirvan Faculty of Pharmacy and Pharmaceutical Sciences 3133 Dentistry/Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada aut Samuel John Faculty of Pharmacy and Pharmaceutical Sciences 3133 Dentistry/Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada aut Lavasanifar Afsaneh Faculty of Pharmacy and Pharmaceutical Sciences 3133 Dentistry/Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/9(2011-09-01), 2288-2301 0724-8741 28:9<2288 2011 28 11095 https://doi.org/10.1007/s11095-011-0459-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-011-0459-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hamdy Samar Faculty of Pharmacy and Pharmaceutical Sciences 3133 Dentistry/Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Haddadi Azita Division of Pharmacy, College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 700 1- Shayeganpour Anooshirvan Faculty of Pharmacy and Pharmaceutical Sciences 3133 Dentistry/Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Samuel John Faculty of Pharmacy and Pharmaceutical Sciences 3133 Dentistry/Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Lavasanifar Afsaneh Faculty of Pharmacy and Pharmaceutical Sciences 3133 Dentistry/Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/9(2011-09-01), 2288-2301 0724-8741 28:9<2288 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer