caa a22 4500 445312270 CHVBK 20180317142634.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s11095-011-0411-z doi (NATIONALLICENCE)springer-10.1007/s11095-011-0411-z Lyophilization of Protein-Loaded Polyelectrolyte Microcapsules [Elektronische Daten] [Marie-Luce De Temmerman, Joanna Rejman, Johan Grooten, Thomas De Beer, Chris Vervaet, Jo Demeester, Stefaan De Smedt] ABSTRACT: Purpose: To evaluate if lyophilization can be used to obtain a dry formulation of polyelectrolyte microcapsules, which have emerged as a new class of microparticles for the encapsulation and delivery of biomacromolecules. Methods: Microcapsules composed of dextran sulfate and poly-L-arginine were obtained by coating CaCO3 microparticles by means of the layer-by-layer technique. Microcapsules were lyophilized using different stabilizers; intactness was checked by CLSM and SEM. Horseradish peroxidase was encapsulated as model enzyme and retained activity after freeze-drying was determined using a fluorescence assay. Ovalbumin was encapsulated as model antigen; immunogenicity after lyophilization was evaluated in vitro by a T-cell proliferation assay and in vivo by measuring the antibody titer in mice. Results: The results clearly demonstrate the necessity of using polyols in the formulation to prevent rupture of the microcapsules and to preserve the activity of encapsulated enzymes. Lyophilized microcapsules appeared as a promising adjuvant for antigen delivery, as both in vitro as in vivo assays showed higher immune activation compared to free antigen. Conclusions: Lyophilization is a promising strategy towards improved stability of protein-loaded microcapsules. Springer Science+Business Media, LLC, 2011 drug delivery nationallicence encapsulation nationallicence layer-by-layer nationallicence lyophilization nationallicence microcapsules nationallicence DEXS : dextran sulfate nationallicence HRP : horseradish peroxidase nationallicence LbL : layer-by-layer nationallicence OVA : ovalbumin nationallicence pARG : poly-L-arginine nationallicence De Temmerman Marie-Luce Laboratory of General Biochemistry and Physical Pharmacy Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium aut Rejman Joanna Laboratory of General Biochemistry and Physical Pharmacy Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium aut Grooten Johan Laboratory of Molecular Immunology Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium aut De Beer Thomas Laboratory of Pharmaceutical Process Analytical Technology Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium aut Vervaet Chris Laboratory of Pharmaceutical Technology Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium aut Demeester Jo Laboratory of General Biochemistry and Physical Pharmacy Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium aut De Smedt Stefaan Laboratory of General Biochemistry and Physical Pharmacy Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/7(2011-07-01), 1765-1773 0724-8741 28:7<1765 2011 28 11095 https://doi.org/10.1007/s11095-011-0411-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-011-0411-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- De Temmerman Marie-Luce Laboratory of General Biochemistry and Physical Pharmacy Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium aut NATIONALLICENCE 700 1- Rejman Joanna Laboratory of General Biochemistry and Physical Pharmacy Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium aut NATIONALLICENCE 700 1- Grooten Johan Laboratory of Molecular Immunology Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium aut NATIONALLICENCE 700 1- De Beer Thomas Laboratory of Pharmaceutical Process Analytical Technology Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium aut NATIONALLICENCE 700 1- Vervaet Chris Laboratory of Pharmaceutical Technology Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium aut NATIONALLICENCE 700 1- Demeester Jo Laboratory of General Biochemistry and Physical Pharmacy Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium aut NATIONALLICENCE 700 1- De Smedt Stefaan Laboratory of General Biochemistry and Physical Pharmacy Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/7(2011-07-01), 1765-1773 0724-8741 28:7<1765 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer