caa a22 4500 445312386 CHVBK 20180317142634.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s11095-011-0453-2 doi (NATIONALLICENCE)springer-10.1007/s11095-011-0453-2 Agarose Surface Coating Influences Intracellular Accumulation and Enhances Payload Stability of a Nano-delivery System [Elektronische Daten] [Enrica De Rosa, Ciro Chiappini, Dongmei Fan, Xuewu Liu, Mauro Ferrari, Ennio Tasciotti] ABSTRACT: Purpose: Protein therapeutics often require repeated administrations of drug over a long period of time. Protein instability is a major obstacle to the development of systems for their controlled and sustained release. We describe a surface modification of nanoporous silicon particles (NSP) with an agarose hydrogel matrix that enhances their ability to load and release proteins, influencing intracellular delivery and preserving molecular stability. Methods: We developed and characterized an agarose surface modification of NSP. Stability of the released protein after enzymatic treatment of loaded particles was evaluated with SDS-page and HPLC analysis. FITC-conjugated BSA was chosen as probe protein and intracellular delivery evaluated by fluorescence microscopy. Results: We showed that agarose coating does not affect NSP protein release rate, while fewer digestion products were found in the released solution after all the enzymatic treatments. Confocal images show that the hydrogel coating improves intracellular delivery, specifically within the nucleus, without affecting the internalization process. Conclusions: This modification of porous silicon adds to its tunability, biocompatibility, and biodegradability the ability to preserve protein integrity during delivery without affecting release rates and internalization dynamics. Moreover, it may allow the silicon particles to function as protein carriers that enable control of cell function. Springer Science+Business Media, LLC, 2011 agarose hydrogel nationallicence intracellular delivery nationallicence nanopores nationallicence porous silicon nationallicence protein nationallicence A1 : agarose composition 0.125% nationallicence A2 : agarose composition 0.05% nationallicence Ag : agarose coated nationallicence APTES : aminopropyltriethoxysilane nationallicence BSA : bovine serum albumin nationallicence FACS : fluorescence activated cell sorting nationallicence FGF : fibroblast growth factor nationallicence HPLC : high performance liquid chromatography nationallicence HUVEC : human umbilical vein endothelial cells nationallicence NC : bare / not coated nationallicence NSPs : nanoporous silicon particles nationallicence PLGA : poly(lactic-co-glycolic acid) nationallicence pSi : porous silicon nationallicence SDS-page : sodium dodecyl sulfate polyacrylamide gel electrophoresis nationallicence SEM : scanning electron microscope nationallicence SiN : silicon nitride nationallicence VEGF : vascular endothelial growth factor nationallicence De Rosa Enrica The Methodist Hospital Research Institute (TMHRI), 6670 Bertner Av# Suite R7-114, 77030, Houston, Texas, USA aut Chiappini Ciro University of Texas at Austin, 10100 Burnet Rd, Bld. 160, 78758, Austin, Texas, USA aut Fan Dongmei The Methodist Hospital Research Institute (TMHRI), 6670 Bertner Av# Suite R7-114, 77030, Houston, Texas, USA aut Liu Xuewu The Methodist Hospital Research Institute (TMHRI), 6670 Bertner Av# Suite R7-114, 77030, Houston, Texas, USA aut Ferrari Mauro The Methodist Hospital Research Institute (TMHRI), 6670 Bertner Av# Suite R7-114, 77030, Houston, Texas, USA aut Tasciotti Ennio The Methodist Hospital Research Institute (TMHRI), 6670 Bertner Av# Suite R7-114, 77030, Houston, Texas, USA aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/7(2011-07-01), 1520-1530 0724-8741 28:7<1520 2011 28 11095 https://doi.org/10.1007/s11095-011-0453-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-011-0453-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- De Rosa Enrica The Methodist Hospital Research Institute (TMHRI), 6670 Bertner Av# Suite R7-114, 77030, Houston, Texas, USA aut NATIONALLICENCE 700 1- Chiappini Ciro University of Texas at Austin, 10100 Burnet Rd, Bld. 160, 78758, Austin, Texas, USA aut NATIONALLICENCE 700 1- Fan Dongmei The Methodist Hospital Research Institute (TMHRI), 6670 Bertner Av# Suite R7-114, 77030, Houston, Texas, USA aut NATIONALLICENCE 700 1- Liu Xuewu The Methodist Hospital Research Institute (TMHRI), 6670 Bertner Av# Suite R7-114, 77030, Houston, Texas, USA aut NATIONALLICENCE 700 1- Ferrari Mauro The Methodist Hospital Research Institute (TMHRI), 6670 Bertner Av# Suite R7-114, 77030, Houston, Texas, USA aut NATIONALLICENCE 700 1- Tasciotti Ennio The Methodist Hospital Research Institute (TMHRI), 6670 Bertner Av# Suite R7-114, 77030, Houston, Texas, USA aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/7(2011-07-01), 1520-1530 0724-8741 28:7<1520 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer