caa a22 4500 44531270X CHVBK 20180317142635.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s11095-011-0501-y doi (NATIONALLICENCE)springer-10.1007/s11095-011-0501-y Treatment of 9L Gliosarcoma in Rats by Ferrociphenol-Loaded Lipid Nanocapsules Based on a Passive Targeting Strategy via the EPR Effect [Elektronische Daten] [Ngoc Huynh, Marie Morille, Jerome Bejaud, Pierre Legras, Anne Vessieres, Gerard Jaouen, Jean-Pierre Benoit, Catherine Passirani] ABSTRACT: Purpose: To study a passive targeting strategy, via the enhanced permeability and retention effect following systemic administration of lipid nanocapsules (LNCs) loaded with ferrociphenol, FcdiOH. Methods: Long chains of polyethylene glycol (DSPE-mPEG2000) were incorporated onto the surface of LNCs by post-insertion technique. Stealth properties of LNCs were investigated by in vitro complement consumption and macrophage uptake, and in vivo pharmacokinetics in healthy rats. Antitumour effect of FcdiOH-loaded LNCs was evaluated in subcutaneous and intracranial 9L gliosarcoma rat models. Results: LNCs and DSPE-mPEG2000-LNCs presented low complement activation and weak macrophage uptake. DSPE-mPEG2000-LNCs exhibited prolonged half-life and extended area under the curve in healthy rats. In a subcutaneous gliosarcoma model, a single intravenous injection of FcdiOH-LNCs (400μL, 2.4mg/rat) considerably inhibited tumour growth when compared to the control. DSPE-mPEG2000-FcdiOH-LNCs exhibited a strong antitumour effect by nearly eradicating the tumour by the end of the study. In intracranial gliosarcoma model, treatment with DSPE-mPEG2000-FcdiOH-LNCs and FcdiOH-LNCs statistically improved median survival time (28 and 27.5days, respectively) compared to the control (25days). Conclusion: These results demonstrate the interesting perspectives for the systemic treatment of glioma thanks to bio-organometallic chemotherapy via lipid nanocapsules. Springer Science+Business Media, LLC, 2011 ectopic nationallicence FcdiOH nationallicence orthotopic nationallicence PEGylated nanoparticles nationallicence stealth properties nationallicence Huynh Ngoc LUNAM Université, Ingénierie de la Vectorisation Particulaire, 49933, Angers, France aut Morille Marie LUNAM Université, Ingénierie de la Vectorisation Particulaire, 49933, Angers, France aut Bejaud Jerome LUNAM Université, Ingénierie de la Vectorisation Particulaire, 49933, Angers, France aut Legras Pierre Service Commun d'Animalerie Hospitalo-Universitaire (SCAHU), 49100, Angers, France aut Vessieres Anne CNRS, UMR 7223, Ecole Nationale Supérieure de Chimie de Paris, 75231, Paris, France aut Jaouen Gerard CNRS, UMR 7223, Ecole Nationale Supérieure de Chimie de Paris, 75231, Paris, France aut Benoit Jean-Pierre LUNAM Université, Ingénierie de la Vectorisation Particulaire, 49933, Angers, France aut Passirani Catherine LUNAM Université, Ingénierie de la Vectorisation Particulaire, 49933, Angers, France aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/12(2011-12-01), 3189-3198 0724-8741 28:12<3189 2011 28 11095 https://doi.org/10.1007/s11095-011-0501-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-011-0501-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huynh Ngoc LUNAM Université, Ingénierie de la Vectorisation Particulaire, 49933, Angers, France aut NATIONALLICENCE 700 1- Morille Marie LUNAM Université, Ingénierie de la Vectorisation Particulaire, 49933, Angers, France aut NATIONALLICENCE 700 1- Bejaud Jerome LUNAM Université, Ingénierie de la Vectorisation Particulaire, 49933, Angers, France aut NATIONALLICENCE 700 1- Legras Pierre Service Commun d'Animalerie Hospitalo-Universitaire (SCAHU), 49100, Angers, France aut NATIONALLICENCE 700 1- Vessieres Anne CNRS, UMR 7223, Ecole Nationale Supérieure de Chimie de Paris, 75231, Paris, France aut NATIONALLICENCE 700 1- Jaouen Gerard CNRS, UMR 7223, Ecole Nationale Supérieure de Chimie de Paris, 75231, Paris, France aut NATIONALLICENCE 700 1- Benoit Jean-Pierre LUNAM Université, Ingénierie de la Vectorisation Particulaire, 49933, Angers, France aut NATIONALLICENCE 700 1- Passirani Catherine LUNAM Université, Ingénierie de la Vectorisation Particulaire, 49933, Angers, France aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/12(2011-12-01), 3189-3198 0724-8741 28:12<3189 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer