caa a22 4500 445312947 CHVBK 20180317142636.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s11095-010-0291-7 doi (NATIONALLICENCE)springer-10.1007/s11095-010-0291-7 In Vivo Methods to Study Uptake of Nanoparticles into the Brain [Elektronische Daten] [Inge van Rooy, Serpil Cakir-Tascioglu, Wim Hennink, Gert Storm, Raymond Schiffelers, Enrico Mastrobattista] ABSTRACT: Several in vivo techniques have been developed to study and measure the uptake of CNS compounds into the brain. With these techniques, various parameters can be determined after drug administration, including the blood-to-brain influx constant (Kin), the permeability-surface area (PS) product, and the brain uptake index (BUI). These techniques have been mostly used for drugs that are expected to enter the brain via transmembrane diffusion or by carrier-mediated transcytosis. Drugs that have limitations in entering the brain via such pathways have been encapsulated in nanoparticles (based on lipids or synthetic polymers) to enhance brain uptake. Nanoparticles are different from CNS compounds in size, composition and uptake mechanisms. This has led to different methods and approaches to study brain uptake in vivo. Here we discuss the techniques generally used to measure nanoparticle uptake in addition to the techniques used for CNS compounds. Techniques include visualization methods, behavioral tests, and quantitative methods. The Author(s), 2010 blood-brain barrier nationallicence brain targeting nationallicence CNS drugs nationallicence nanoparticles nationallicence van Rooy Inge Department of Pharmaceutics Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box80082, 3508 TB, Utrecht, The Netherlands aut Cakir-Tascioglu Serpil Unilever R&D, Olivier van Noortlaan 120, 3133 AT, Vlaardingen, The Netherlands aut Hennink Wim Department of Pharmaceutics Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box80082, 3508 TB, Utrecht, The Netherlands aut Storm Gert Department of Pharmaceutics Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box80082, 3508 TB, Utrecht, The Netherlands aut Schiffelers Raymond Department of Pharmaceutics Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box80082, 3508 TB, Utrecht, The Netherlands aut Mastrobattista Enrico Department of Pharmaceutics Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box80082, 3508 TB, Utrecht, The Netherlands aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/3(2011-03-01), 456-471 0724-8741 28:3<456 2011 28 11095 https://doi.org/10.1007/s11095-010-0291-7 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-010-0291-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van Rooy Inge Department of Pharmaceutics Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box80082, 3508 TB, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Cakir-Tascioglu Serpil Unilever R&D, Olivier van Noortlaan 120, 3133 AT, Vlaardingen, The Netherlands aut NATIONALLICENCE 700 1- Hennink Wim Department of Pharmaceutics Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box80082, 3508 TB, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Storm Gert Department of Pharmaceutics Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box80082, 3508 TB, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Schiffelers Raymond Department of Pharmaceutics Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box80082, 3508 TB, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Mastrobattista Enrico Department of Pharmaceutics Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box80082, 3508 TB, Utrecht, The Netherlands aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/3(2011-03-01), 456-471 0724-8741 28:3<456 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer