caa a22 4500 445313161 CHVBK 20180317142637.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s11095-011-0469-7 doi (NATIONALLICENCE)springer-10.1007/s11095-011-0469-7 Identification of a LNCaP-Specific Binding Peptide Using Phage Display [Elektronische Daten] [Bin Qin, Wanyi Tai, Ravi Shukla, Kun Cheng] ABSTRACT: Purpose: To identify a LNCaP-specific peptide using a phage display library and evaluate its potential applications in targeted drug delivery. Methods: Binding abilities of selected phages were evaluated by cell phage ELISA. The KYL peptide encoded by the most specific phage clone was synthesized, labeled with fluorescein, and assayed in various cell lines. A fusion peptide composed of the KYL peptide and a proapoptotic peptide D(KLAKLAK)2 was synthesized, and the cell death effect was evaluated on different cells. Moreover, the KYL peptide was conjugated to a cationic protein, protamine, to explore its potential application in siRNA delivery. Results: One phage clone with a high binding affinity to LNCaP cells was identified. Cell phage ELISA and immunostaining demonstrated high specificity of this phage to LNCaP cells. The fluorescein-labeled KYL peptide exhibited higher binding to LNCaP cells in comparison to other cells. The fusion peptide composed of the KYL peptide and the proapoptotic peptide induced cell death in LNCaP cells, but not in PC-3 cells. The KYL peptide-protamine conjugate also efficiently delivered a fluorescein-labeled siRNA into LNCaP cells. Conclusion: We identified a LNCaP-specific peptide and demonstrated its potential applications in targeted drug delivery to LNCaP cells. Springer Science+Business Media, LLC, 2011 LNCaP nationallicence peptide ligand nationallicence phage display nationallicence prostate cancer nationallicence siRNA nationallicence Qin Bin Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City, 2464 Charlotte Street, 64108, Kansas City, Missouri, USA aut Tai Wanyi Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City, 2464 Charlotte Street, 64108, Kansas City, Missouri, USA aut Shukla Ravi Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City, 2464 Charlotte Street, 64108, Kansas City, Missouri, USA aut Cheng Kun Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City, 2464 Charlotte Street, 64108, Kansas City, Missouri, USA aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/10(2011-10-01), 2422-2434 0724-8741 28:10<2422 2011 28 11095 https://doi.org/10.1007/s11095-011-0469-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-011-0469-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Qin Bin Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City, 2464 Charlotte Street, 64108, Kansas City, Missouri, USA aut NATIONALLICENCE 700 1- Tai Wanyi Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City, 2464 Charlotte Street, 64108, Kansas City, Missouri, USA aut NATIONALLICENCE 700 1- Shukla Ravi Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City, 2464 Charlotte Street, 64108, Kansas City, Missouri, USA aut NATIONALLICENCE 700 1- Cheng Kun Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City, 2464 Charlotte Street, 64108, Kansas City, Missouri, USA aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/10(2011-10-01), 2422-2434 0724-8741 28:10<2422 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer