caa a22 4500 445313323 CHVBK 20180317142637.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s11095-010-0346-9 doi (NATIONALLICENCE)springer-10.1007/s11095-010-0346-9 Paclitaxel-Loaded Poly(n-butylcyanoacrylate) Nanoparticle Delivery System to Overcome Multidrug Resistance in Ovarian Cancer [Elektronische Daten] [Fei Ren, Ruda Chen, Ying Wang, Yabin Sun, Yaodong Jiang, Guofeng Li] ABSTRACT: Purpose: The aim of this study was to test the ability of paclitaxel-loaded poly(butylcyanoacrylate) (PBCA) nanoparticles to overcome multidrug resistance (MDR) in human ovarian resistant cells (A2780/T) and investigate its possible mechanism. Methods: We prepared paclitaxel-loaded PBCA nanoparticles by interfacial polymerization method. The physicochemistry of the nanoparticles was characterized. The cytotoxicity of paclitaxel-loaded PBCA nanoparticles was measured by MTT assay. Calcein-AM assay was used to analyze the P-glycoprotein (P-gp) function, and the expression of MDR-1 mRNA in A2780/T cells treated with drug-loaded nanoparticles was defined by QRT-PCR. Results: The nanoparticles were approximately spherical in shape with an average diameter of 224.5 ± 5.7nm. The encapsulation efficiency was 99.23%. The in vitro drug release profile exhibited a biphasic pattern. The drug formulated in PBCA nanoparticles showed a greater cytotoxicity than paclitaxel against A2780/T cells. Paclitaxel-loaded PBCA as well as blank PBCA nanoparticles decreased P-gp function in a dose-dependent manner, suggesting the efficacy of the drug-loaded nanoparticle system on overcoming MDR. There was no significant effect on inhibition to the expression of MDR1 mRNA. Conclusions: Paclitaxel-loaded PBCA nanoparticles can enhance cytotoxicity and overcome MDR through a mechanism of the inhibition of P-gp function caused by the nanoparticles system. Springer Science+Business Media, LLC, 2010 multidrug resistance nationallicence nanoparticles nationallicence paclitaxel nationallicence P-glycoprotein function nationallicence P-glycoprotein gene expression nationallicence Ren Fei Department of Pharmacy, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut Chen Ruda Department of Pharmacy, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut Wang Ying Institute of Cancer Research,, Southern Medical University, 510515, Guangzhou, China aut Sun Yabin Department of Pharmacy, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut Jiang Yaodong Department of Urology, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut Li Guofeng Department of Pharmacy, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/4(2011-04-01), 897-906 0724-8741 28:4<897 2011 28 11095 https://doi.org/10.1007/s11095-010-0346-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-010-0346-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ren Fei Department of Pharmacy, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Chen Ruda Department of Pharmacy, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Wang Ying Institute of Cancer Research,, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Sun Yabin Department of Pharmacy, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Jiang Yaodong Department of Urology, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Li Guofeng Department of Pharmacy, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/4(2011-04-01), 897-906 0724-8741 28:4<897 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer