caa a22 4500 445313366 CHVBK 20180317142637.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s11095-011-0366-0 doi (NATIONALLICENCE)springer-10.1007/s11095-011-0366-0 Evaluation of Osteoclastogenesis via NFκB Decoy/mannosylated Cationic Liposome-Mediated Inhibition of Pro-inflammatory Cytokine Production from Primary Cultured Macrophages [Elektronische Daten] [Thuy Dinh, Yuriko Higuchi, Shigeru Kawakami, Fumiyoshi Yamashita, Mitsuru Hashida] ABSTRACT: Purpose: To explore the effect of NFκB activation in macrophages on osteoclastogenesis of bone marrow cells for potential application as a new type of therapy for preventing bone loss. Methods: Primary cultured macrophages and bone marrow cells were prepared from mice. As macrophage-targeted carriers, Mannosylated cationic liposomes (Man-liposomes) were prepared and were allowed to form complexes with NFκB decoy (a double-stranded oligonucleotide). Cellular uptake, inhibition of NFκB activation, and cytokine production were evaluated using macrophages. Osteoclastogenesis was investigated using bone marrow cells, which were cultured in the conditioned medium prepared from macrophages with or without Man-liposome/NFκB decoy complexes treatment. Results: Cellular accumulation of NFκB decoy was enhanced by Man-liposome. NFκB activation in macrophages and TNF-α production were suppressed in macrophages by Man-liposome/NFκB decoy complexes but not by the naked NFκB decoy, Gal-liposome/NFκB decoy complexes, or Man-liposome/random decoy complexes. Osteoclastogenesis of bone marrow cells was induced in the conditioned medium prepared from activated macrophages but not by activated macrophages treated with Man-liposome/NFκB decoy complexes. Conclusion: Osteoclastogenesis induced by activated macrophages could be suppressed by the treatment macrophages with Man-liposome/NFκB decoy complexes. Macrophage-targeted delivery of NFκB decoys using Man-liposomes may be promising in its use for the remediation of bone loss. Springer Science+Business Media, LLC, 2011 man-liposomes nationallicence NFκB decoy nationallicence osteoclastogenesis nationallicence proinflammatory cytokines nationallicence Man-liposome : mannosylated cationic liposome nationallicence NFκB : nuclea factor kappa B nationallicence RA : rheumatoid artitis nationallicence RANKL : receptor activator NF-kappaB ligand nationallicence TNFα : tumor necrosis factor alpha nationallicence Dinh Thuy Department of Drug Delivery Research Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29, Yoshida-shimoadachi, Sakyo-ku, 606-8501, Kyoto, Japan aut Higuchi Yuriko Department of Drug Delivery Research Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29, Yoshida-shimoadachi, Sakyo-ku, 606-8501, Kyoto, Japan aut Kawakami Shigeru Department of Drug Delivery Research Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29, Yoshida-shimoadachi, Sakyo-ku, 606-8501, Kyoto, Japan aut Yamashita Fumiyoshi Department of Drug Delivery Research Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29, Yoshida-shimoadachi, Sakyo-ku, 606-8501, Kyoto, Japan aut Hashida Mitsuru Department of Drug Delivery Research Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29, Yoshida-shimoadachi, Sakyo-ku, 606-8501, Kyoto, Japan aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/4(2011-04-01), 742-751 0724-8741 28:4<742 2011 28 11095 https://doi.org/10.1007/s11095-011-0366-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-011-0366-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dinh Thuy Department of Drug Delivery Research Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29, Yoshida-shimoadachi, Sakyo-ku, 606-8501, Kyoto, Japan aut NATIONALLICENCE 700 1- Higuchi Yuriko Department of Drug Delivery Research Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29, Yoshida-shimoadachi, Sakyo-ku, 606-8501, Kyoto, Japan aut NATIONALLICENCE 700 1- Kawakami Shigeru Department of Drug Delivery Research Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29, Yoshida-shimoadachi, Sakyo-ku, 606-8501, Kyoto, Japan aut NATIONALLICENCE 700 1- Yamashita Fumiyoshi Department of Drug Delivery Research Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29, Yoshida-shimoadachi, Sakyo-ku, 606-8501, Kyoto, Japan aut NATIONALLICENCE 700 1- Hashida Mitsuru Department of Drug Delivery Research Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29, Yoshida-shimoadachi, Sakyo-ku, 606-8501, Kyoto, Japan aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/4(2011-04-01), 742-751 0724-8741 28:4<742 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer