caa a22 4500 445313382 CHVBK 20180317142638.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s11095-010-0338-9 doi (NATIONALLICENCE)springer-10.1007/s11095-010-0338-9 Hydrodynamic Gene Delivery and Its Applications in Pharmaceutical Research [Elektronische Daten] [Barbara Bonamassa, Li Hai, Dexi Liu] ABSTRACT: Hydrodynamic delivery has emerged as the simplest and most effective method for intracellular delivery of membrane-impermeable substances in rodents. The system employs a physical force generated by a rapid injection of large volume of solution into a blood vessel to enhance the permeability of endothelium and the plasma membrane of the parenchyma cells to allow delivery of substance into cells. The procedure was initially established for gene delivery in mice, and its applications have been extended to the delivery of proteins, oligo nucleotides, genomic DNA and RNA sequences, and small molecules. The focus of this review is on applications of hydrodynamic delivery in pharmaceutical research. Examples are provided to highlight the use of hydrodynamic delivery for study of transcriptional regulation of CYP enzymes, for establishment of animal model for viral infections, and for gene drug discovery and gene function analysis. Springer Science+Business Media, LLC, 2010 gene therapy nationallicence hydrodynamic delivery nationallicence nonviral gene delivery nationallicence oligo nucleotides nationallicence protein drug discovery nationallicence siRNA nationallicence Bonamassa Barbara Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, 527 Salk Hall, 3501 Terrace Street, 15261, Pittsburgh, Pennsylvania, USA aut Hai Li Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, 527 Salk Hall, 3501 Terrace Street, 15261, Pittsburgh, Pennsylvania, USA aut Liu Dexi Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, 527 Salk Hall, 3501 Terrace Street, 15261, Pittsburgh, Pennsylvania, USA aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/4(2011-04-01), 694-701 0724-8741 28:4<694 2011 28 11095 https://doi.org/10.1007/s11095-010-0338-9 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-010-0338-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bonamassa Barbara Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, 527 Salk Hall, 3501 Terrace Street, 15261, Pittsburgh, Pennsylvania, USA aut NATIONALLICENCE 700 1- Hai Li Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, 527 Salk Hall, 3501 Terrace Street, 15261, Pittsburgh, Pennsylvania, USA aut NATIONALLICENCE 700 1- Liu Dexi Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, 527 Salk Hall, 3501 Terrace Street, 15261, Pittsburgh, Pennsylvania, USA aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/4(2011-04-01), 694-701 0724-8741 28:4<694 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer