caa a22 4500 445313404 CHVBK 20180317142638.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s11095-010-0286-4 doi (NATIONALLICENCE)springer-10.1007/s11095-010-0286-4 Delivery of an Angiogenic Gene into Ischemic Muscle by Novel Bubble Liposomes Followed by Ultrasound Exposure [Elektronische Daten] [Yoichi Negishi, Keiko Matsuo, Yoko Endo-Takahashi, Kentaro Suzuki, Yuuki Matsuki, Norio Takagi, Ryo Suzuki, Kazuo Maruyama, Yukihiko Aramaki] ABSTRACT: Purpose: To develop a safe and efficient gene delivery system into skeletal muscle using the combination of Bubble liposomes (BL) and ultrasound (US) exposure, and to assess the feasibility and the effectiveness of BL for angiogenic gene delivery in clinical use. Methods: A solution of luciferase-expressing plasmid DNA (pDNA) and BL was injected into the tibialis (TA) muscle, and US was immediately applied to the injection site. The transfection efficiency was estimated by a luciferase assay. The ischemic hindlimb was also treated with BL and US-mediated intramuscular gene transfer of bFGF-expressing plasmid DNA. Capillary vessels were assessed using immunostaining. The blood flow was determined using a laser Doppler blood flow meter. Results: Highly efficient gene transfer could be achieved in the muscle transfected with BLs, and US mediated the gene transfer. Capillary vessels were enhanced in the treatment groups with this gene transfer method. The blood flow in the treated groups with this gene transfer method quickly recovered compared to other treatment groups (non-treated, bFGF alone, or bFGF+US). Conclusion: The gene transfer system into skeletal muscle using the combination of BL and US exposure could be an effective means for angiogenic gene therapy in limb ischemia. Springer Science+Business Media, LLC, 2010 angiogenesis nationallicence bubble liposomes nationallicence gene delivery nationallicence ultrasound nationallicence Negishi Yoichi Department of Drug and Gene Delivery Systems School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut Matsuo Keiko Department of Drug and Gene Delivery Systems School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut Endo-Takahashi Yoko Department of Drug and Gene Delivery Systems School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut Suzuki Kentaro Department of Drug and Gene Delivery Systems School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut Matsuki Yuuki Department of Drug and Gene Delivery Systems School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut Takagi Norio Department of Molecular and Cellular Pharmacology School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut Suzuki Ryo Department of Pharmaceutics, Teikyo University, 1091-1 Suwarashi, Midori-ku, 252-5195, Sagamihara, Kanagawa, Japan aut Maruyama Kazuo Department of Pharmaceutics, Teikyo University, 1091-1 Suwarashi, Midori-ku, 252-5195, Sagamihara, Kanagawa, Japan aut Aramaki Yukihiko Department of Drug and Gene Delivery Systems School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/4(2011-04-01), 712-719 0724-8741 28:4<712 2011 28 11095 https://doi.org/10.1007/s11095-010-0286-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-010-0286-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Negishi Yoichi Department of Drug and Gene Delivery Systems School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut NATIONALLICENCE 700 1- Matsuo Keiko Department of Drug and Gene Delivery Systems School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut NATIONALLICENCE 700 1- Endo-Takahashi Yoko Department of Drug and Gene Delivery Systems School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut NATIONALLICENCE 700 1- Suzuki Kentaro Department of Drug and Gene Delivery Systems School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut NATIONALLICENCE 700 1- Matsuki Yuuki Department of Drug and Gene Delivery Systems School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut NATIONALLICENCE 700 1- Takagi Norio Department of Molecular and Cellular Pharmacology School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut NATIONALLICENCE 700 1- Suzuki Ryo Department of Pharmaceutics, Teikyo University, 1091-1 Suwarashi, Midori-ku, 252-5195, Sagamihara, Kanagawa, Japan aut NATIONALLICENCE 700 1- Maruyama Kazuo Department of Pharmaceutics, Teikyo University, 1091-1 Suwarashi, Midori-ku, 252-5195, Sagamihara, Kanagawa, Japan aut NATIONALLICENCE 700 1- Aramaki Yukihiko Department of Drug and Gene Delivery Systems School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/4(2011-04-01), 712-719 0724-8741 28:4<712 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer