caa a22 4500 445313560 CHVBK 20180317142638.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s11095-011-0425-6 doi (NATIONALLICENCE)springer-10.1007/s11095-011-0425-6 Poly(Lactide-co-Glycolide) Nanocapsules Containing Benzocaine: Influence of the Composition of the Oily Nucleus on Physico-Chemical Properties and Anesthetic Activity [Elektronische Daten] [Nathalie de Melo, Renato Grillo, Viviane Guilherme, Daniele de Araujo, Eneida de Paula, André Rosa, Leonardo Fraceto] ABSTRACT: Purpose: The aim of this work was to investigate the influence of the oily nucleus composition on physico-chemical properties and anesthetic activity of poly (lactide-co-glycolide) nanocapsules with benzocaine. Methods: Nanocapsules containing benzocaine were prepared with three different oily nucleus composition and characterized by mean diameter, polydispersivity, zeta potential, pH and stability were investigated as a function of time. In vitro release kinetics were performed in a system with two compartments separated by a cellulose membrane. Intensity and duration of analgesia were evaluated in rats by sciatic nerve blockade. Results: The greatest stability, slower release profile and improvement in the local anesthetic activity of BZC were obtained with the formulation using USP mineral oil as component. Conclusions: Results from our study provide useful perspectives on selection of the primary materials needed to produce suspensions of polymeric nanocapsules able to act as carriers of BZC, with potential future application in the treatment of pain. Springer Science+Business Media, LLC, 2011 benzocaine nationallicence local anesthetic nationallicence nanocapsule nationallicence oily nucleus nationallicence PLGA nationallicence de Melo Nathalie Department of Environmental Engineering, São Paulo State University (UNESP), Av. Três de Março, 511, 18087-180, Sorocaba, SP, Brazil aut Grillo Renato Department of Environmental Engineering, São Paulo State University (UNESP), Av. Três de Março, 511, 18087-180, Sorocaba, SP, Brazil aut Guilherme Viviane Department of Biochemistry, State University of Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil aut de Araujo Daniele Department of Biochemistry, State University of Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil aut de Paula Eneida Department of Biochemistry, State University of Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil aut Rosa André Department of Environmental Engineering, São Paulo State University (UNESP), Av. Três de Março, 511, 18087-180, Sorocaba, SP, Brazil aut Fraceto Leonardo Department of Environmental Engineering, São Paulo State University (UNESP), Av. Três de Março, 511, 18087-180, Sorocaba, SP, Brazil aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/8(2011-08-01), 1984-1994 0724-8741 28:8<1984 2011 28 11095 https://doi.org/10.1007/s11095-011-0425-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-011-0425-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Melo Nathalie Department of Environmental Engineering, São Paulo State University (UNESP), Av. Três de Março, 511, 18087-180, Sorocaba, SP, Brazil aut NATIONALLICENCE 700 1- Grillo Renato Department of Environmental Engineering, São Paulo State University (UNESP), Av. Três de Março, 511, 18087-180, Sorocaba, SP, Brazil aut NATIONALLICENCE 700 1- Guilherme Viviane Department of Biochemistry, State University of Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil aut NATIONALLICENCE 700 1- de Araujo Daniele Department of Biochemistry, State University of Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil aut NATIONALLICENCE 700 1- de Paula Eneida Department of Biochemistry, State University of Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil aut NATIONALLICENCE 700 1- Rosa André Department of Environmental Engineering, São Paulo State University (UNESP), Av. Três de Março, 511, 18087-180, Sorocaba, SP, Brazil aut NATIONALLICENCE 700 1- Fraceto Leonardo Department of Environmental Engineering, São Paulo State University (UNESP), Av. Três de Março, 511, 18087-180, Sorocaba, SP, Brazil aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/8(2011-08-01), 1984-1994 0724-8741 28:8<1984 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer