caa a22 4500 445313919 CHVBK 20180317142639.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s11095-010-0361-x doi (NATIONALLICENCE)springer-10.1007/s11095-010-0361-x Biomaterial-Based Vaccine Induces Regression of Established Intracranial Glioma in Rats [Elektronische Daten] [Omar Ali, Ed Doherty, William Bell, Tracie Fradet, Jebecka Hudak, Marie-Therese Laliberte, David Mooney, Dwaine Emerich] ABSTRACT: Purpose: The prognosis for glioma patients is poor, and development of new treatments is critical. Previously, we engineered polymer-based vaccines that control GM-CSF, CpG-oligonucleotide, and tumor-lysate presentation to regulate immune cell trafficking and activation, which promoted potent immune responses against peripheral tumors. Here, we extend the use of this system to glioma. Methods: Rats were challenged with an intracranial injection of glioma cells followed (1week) by administration of the polymeric vaccine (containing GM-CSF, CpG, and tumor-lysate) in the tumor bed. Control rats were treated with blank matrices, matrices with GM-CSF and CpG, or intra-tumoral bolus injections of GM-CSF, CpG, and tumor lysate. Rats were monitored for survival and tested for neurological function. Results: Survival studies confirmed a benefit of the polymeric vaccine as 90% of vaccinated rats survived for >100days. Control rats exhibited minimal benefit. Motor tests revealed that vaccination protected against the loss of forelimb use produced by glioma growth. Histological analysis quantitatively confirmed a robust and rapid reduction in tumor size. Long-term immunity was confirmed when 67% of survivors also survived a second glioma challenge. Conclusions: These studies extend previous reports regarding this approach to tumor therapy and justify further development for glioma treatment. Springer Science+Business Media, LLC, 2011 brain tumor nationallicence cancer vaccine nationallicence glioma nationallicence immunotherapy nationallicence PLG nationallicence Ali Omar InCytu, Inc., 701 George Washington Highway, 02865, Lincoln, Rhode Island, USA aut Doherty Ed InCytu, Inc., 701 George Washington Highway, 02865, Lincoln, Rhode Island, USA aut Bell William InCytu, Inc., 701 George Washington Highway, 02865, Lincoln, Rhode Island, USA aut Fradet Tracie InCytu, Inc., 701 George Washington Highway, 02865, Lincoln, Rhode Island, USA aut Hudak Jebecka InCytu, Inc., 701 George Washington Highway, 02865, Lincoln, Rhode Island, USA aut Laliberte Marie-Therese InCytu, Inc., 701 George Washington Highway, 02865, Lincoln, Rhode Island, USA aut Mooney David InCytu, Inc., 701 George Washington Highway, 02865, Lincoln, Rhode Island, USA aut Emerich Dwaine InCytu, Inc., 701 George Washington Highway, 02865, Lincoln, Rhode Island, USA aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/5(2011-05-01), 1074-1080 0724-8741 28:5<1074 2011 28 11095 https://doi.org/10.1007/s11095-010-0361-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-010-0361-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ali Omar InCytu, Inc., 701 George Washington Highway, 02865, Lincoln, Rhode Island, USA aut NATIONALLICENCE 700 1- Doherty Ed InCytu, Inc., 701 George Washington Highway, 02865, Lincoln, Rhode Island, USA aut NATIONALLICENCE 700 1- Bell William InCytu, Inc., 701 George Washington Highway, 02865, Lincoln, Rhode Island, USA aut NATIONALLICENCE 700 1- Fradet Tracie InCytu, Inc., 701 George Washington Highway, 02865, Lincoln, Rhode Island, USA aut NATIONALLICENCE 700 1- Hudak Jebecka InCytu, Inc., 701 George Washington Highway, 02865, Lincoln, Rhode Island, USA aut NATIONALLICENCE 700 1- Laliberte Marie-Therese InCytu, Inc., 701 George Washington Highway, 02865, Lincoln, Rhode Island, USA aut NATIONALLICENCE 700 1- Mooney David InCytu, Inc., 701 George Washington Highway, 02865, Lincoln, Rhode Island, USA aut NATIONALLICENCE 700 1- Emerich Dwaine InCytu, Inc., 701 George Washington Highway, 02865, Lincoln, Rhode Island, USA aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/5(2011-05-01), 1074-1080 0724-8741 28:5<1074 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer