caa a22 4500 445313986 CHVBK 20180317142639.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s11095-010-0349-6 doi (NATIONALLICENCE)springer-10.1007/s11095-010-0349-6 Influence of Acylation on the Adsorption of Insulin to Hydrophobic Surfaces [Elektronische Daten] [Charlotte Pinholt, Susanne Hostrup, Jens Bukrinsky, Sven Frokjaer, Lene Jorgensen] ABSTRACT: Purpose: To study the effect of acylation on the adsorption of insulin to hydrophobic polystyrene beads. Methods: Adsorption isotherms for adsorption of insulin and acylated insulin to hydrophobic polystyrene beads were established, and the adsorption of the two proteins was compared further with isothermal titration calorimetry. In addition, the secondary structure and the association behavior of the two proteins were studied with circular dichroism. Results: Insulin and acylated insulin adsorbed with high affinity to the hydrophobic polystyrene beads. More acylated insulin molecules than insulin molecules adsorbed per unit surface area from solutions containing monomer-dimer mixtures of acylated insulin and insulin, respectively. In contrast, no difference was observed in the number of insulin and acylated insulin molecules adsorbing per unit surface area, when adsorption occurred from solutions containing monomer-dimer-hexamer mixtures. Conclusion: The influence of acylation on the adsorption behavior of insulin depends on the association degree of insulin, possibly due to a greater difference in hydrophobicity between monomeric insulin and acylated insulin than between the hexameric forms of these two proteins. Springer Science+Business Media, LLC, 2010 acylated insulin nationallicence insulin nationallicence protein adsorption nationallicence ITC nationallicence CD nationallicence Pinholt Charlotte Department of Pharmaceutics and Analytical Chemistry Faculty of Pharmaceutical Sciences, University of Copenhagen, DK-2100, Copenhagen, Denmark aut Hostrup Susanne CMC Diabetes Analysis and Formulation, Novo Nordisk A/S, DK-2880, Bagsvaerd, Denmark aut Bukrinsky Jens Novozymes Biopharma DK A/S, DK-2880, Bagsvaerd, Denmark aut Frokjaer Sven Department of Pharmaceutics and Analytical Chemistry Faculty of Pharmaceutical Sciences, University of Copenhagen, DK-2100, Copenhagen, Denmark aut Jorgensen Lene Department of Pharmaceutics and Analytical Chemistry Faculty of Pharmaceutical Sciences, University of Copenhagen, DK-2100, Copenhagen, Denmark aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/5(2011-05-01), 1031-1040 0724-8741 28:5<1031 2011 28 11095 https://doi.org/10.1007/s11095-010-0349-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-010-0349-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pinholt Charlotte Department of Pharmaceutics and Analytical Chemistry Faculty of Pharmaceutical Sciences, University of Copenhagen, DK-2100, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Hostrup Susanne CMC Diabetes Analysis and Formulation, Novo Nordisk A/S, DK-2880, Bagsvaerd, Denmark aut NATIONALLICENCE 700 1- Bukrinsky Jens Novozymes Biopharma DK A/S, DK-2880, Bagsvaerd, Denmark aut NATIONALLICENCE 700 1- Frokjaer Sven Department of Pharmaceutics and Analytical Chemistry Faculty of Pharmaceutical Sciences, University of Copenhagen, DK-2100, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Jorgensen Lene Department of Pharmaceutics and Analytical Chemistry Faculty of Pharmaceutical Sciences, University of Copenhagen, DK-2100, Copenhagen, Denmark aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/5(2011-05-01), 1031-1040 0724-8741 28:5<1031 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer