caa a22 4500 44531401X CHVBK 20180317142640.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s11095-010-0248-x doi (NATIONALLICENCE)springer-10.1007/s11095-010-0248-x High Loading of Gentamicin in Bioadhesive PVM/MA Nanostructured Microparticles Using Compressed Carbon-Dioxide [Elektronische Daten] [Elisa Elizondo, Santiago Sala, Edurne Imbuluzqueta, David González, María Blanco-Prieto, Carlos Gamazo, Nora Ventosa, Jaume Veciana] ABSTRACT: Purpose: To investigate, for the first time, the viability of compressed antisolvent methodologies for the preparation of drug-loaded particles of the biodegradable and bioadhesive polymer poly (methyl vinyl ether-co-maleic anhydride) (PVM/MA), utilizing gentamicin (Gm) as a model drug. Methods: Precipitation with a Compressed Antisolvent (PCA) method was used for the preparation of PVM/MA particles loaded with gentamicin. Before encapsulation, gentamicin was modified into a hydrophobic complex, GmAOT, by exchanging its sulphate ions with an anionic surfactant. GmAOT:PVM/MA composites were fully characterized in terms of size, morphology, composition, drug distribution, phase composition, in vitro activity and drug release. Results: Homogeneous nanostructured microparticles of PVM/MA loaded with high and uniformly distributed quantities of GmAOT were obtained by PCA. The drug loading factors could be tuned at will, improving up to ten times the loadings obtained by other precipitation techniques. Gentamicin retained its bioactivity after being processed, and, according to its release profiles, after an initial burst it experienced a sustained release over 30days. Conclusions: Compressed antisolvent methods are suitable technologies for the one-step preparation of highly loaded nanostructured PVM/MA matrices with promising application in the delivery of low bioavailable drugs. Springer Science+Business Media, LLC, 2010 Gantrez AN nationallicence gentamicin nationallicence microparticles nationallicence PVM/MA nationallicence supercritical fluids nationallicence Elizondo Elisa Department of Molecular Nanoscience and Organic Materials, Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), 08193, Bellaterra, Spain aut Sala Santiago Department of Molecular Nanoscience and Organic Materials, Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), 08193, Bellaterra, Spain aut Imbuluzqueta Edurne Department of Pharmacy and Pharmaceutical Technology, University of Navarra, 31080, Pamplona, Spain aut González David Department of Pharmacy and Pharmaceutical Technology, University of Navarra, 31080, Pamplona, Spain aut Blanco-Prieto María Department of Pharmacy and Pharmaceutical Technology, University of Navarra, 31080, Pamplona, Spain aut Gamazo Carlos Department of Microbiology, University of Navarra, 31080, Pamplona, Spain aut Ventosa Nora Department of Molecular Nanoscience and Organic Materials, Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), 08193, Bellaterra, Spain aut Veciana Jaume Department of Molecular Nanoscience and Organic Materials, Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), 08193, Bellaterra, Spain aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/2(2011-02-01), 309-321 0724-8741 28:2<309 2011 28 11095 https://doi.org/10.1007/s11095-010-0248-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-010-0248-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Elizondo Elisa Department of Molecular Nanoscience and Organic Materials, Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), 08193, Bellaterra, Spain aut NATIONALLICENCE 700 1- Sala Santiago Department of Molecular Nanoscience and Organic Materials, Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), 08193, Bellaterra, Spain aut NATIONALLICENCE 700 1- Imbuluzqueta Edurne Department of Pharmacy and Pharmaceutical Technology, University of Navarra, 31080, Pamplona, Spain aut NATIONALLICENCE 700 1- González David Department of Pharmacy and Pharmaceutical Technology, University of Navarra, 31080, Pamplona, Spain aut NATIONALLICENCE 700 1- Blanco-Prieto María Department of Pharmacy and Pharmaceutical Technology, University of Navarra, 31080, Pamplona, Spain aut NATIONALLICENCE 700 1- Gamazo Carlos Department of Microbiology, University of Navarra, 31080, Pamplona, Spain aut NATIONALLICENCE 700 1- Ventosa Nora Department of Molecular Nanoscience and Organic Materials, Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), 08193, Bellaterra, Spain aut NATIONALLICENCE 700 1- Veciana Jaume Department of Molecular Nanoscience and Organic Materials, Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), 08193, Bellaterra, Spain aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/2(2011-02-01), 309-321 0724-8741 28:2<309 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer