caa a22 4500 445314079 CHVBK 20180317142640.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s11095-010-0276-6 doi (NATIONALLICENCE)springer-10.1007/s11095-010-0276-6 An Effective Strategy for the Synthesis of Biocompatible Gold Nanoparticles Using Cinnamon Phytochemicals for Phantom CT Imaging and Photoacoustic Detection of Cancerous Cells [Elektronische Daten] [Nripen Chanda, Ravi Shukla, Ajit Zambre, Swapna Mekapothula, Rajesh Kulkarni, Kavita Katti, Kiran Bhattacharyya, Genevieve Fent, Stan Casteel, Evan Boote, John Viator, Anandhi Upendran, Raghuraman Kannan, Kattesh Katti] ABSTRACT: Purpose: The purpose of the present study was to explore the utilization of cinnamon-coated gold nanoparticles (Cin-AuNPs) as CT/optical contrast-enhancement agents for detection of cancer cells. Methods: Cin-AuNPs were synthesized by a "green” procedure, and the detailed characterization was performed by physico-chemical analysis. Cytotoxicity and cellular uptake studies were carried out in normal human fibroblast and cancerous (PC-3 and MCF-7) cells, respectively. The efficacy of detecting cancerous cells was monitored using a photoacoustic technique. In vivo biodistribution was studied after IV injection of Cin-AuNPs in mice, and also a CT phantom model was generated. Results: Biocompatible Cin-AuNPs were synthesized with high purity. Significant uptake of these gold nanoparticles was observed in PC-3 and MCF-7 cells. Cin-AuNPs internalized in cancerous cells facilitated detectable photoacoustic signals. In vivo biodistribution in normal mice showed steady accumulation of gold nanoparticles in lungs and rapid clearance from blood. Quantitative analysis of CT values in phantom model revealed that the cinnamon-phytochemical-coated AuNPs have reasonable attenuation efficiency. Conclusions: The results indicate that these non-toxic Cin-AuNPs can serve as excellent CT/ photoacoustic contrast-enhancement agents and may provide a novel approach toward tumor detection through nanopharmaceuticals. Springer Science+Business Media, LLC, 2010 cancer cells nationallicence cellular internalization nationallicence cinnamon-stabilized gold nanoparticles nationallicence in vivo biodistribution nationallicence phantom CT imaging nationallicence photoacoustic detection nationallicence Chanda Nripen Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut Shukla Ravi Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut Zambre Ajit Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut Mekapothula Swapna Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut Kulkarni Rajesh Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut Katti Kavita Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut Bhattacharyya Kiran Department of Biological Engineering, University of Missouri, Columbia, Missouri, USA aut Fent Genevieve Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA aut Casteel Stan Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA aut Boote Evan Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut Viator John Department of Biological Engineering, University of Missouri, Columbia, Missouri, USA aut Upendran Anandhi Nanoparticle Biochem, Inc., 65201, Columbia, Missouri, USA aut Kannan Raghuraman Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut Katti Kattesh Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/2(2011-02-01), 279-291 0724-8741 28:2<279 2011 28 11095 https://doi.org/10.1007/s11095-010-0276-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-010-0276-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chanda Nripen Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut NATIONALLICENCE 700 1- Shukla Ravi Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut NATIONALLICENCE 700 1- Zambre Ajit Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut NATIONALLICENCE 700 1- Mekapothula Swapna Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut NATIONALLICENCE 700 1- Kulkarni Rajesh Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut NATIONALLICENCE 700 1- Katti Kavita Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut NATIONALLICENCE 700 1- Bhattacharyya Kiran Department of Biological Engineering, University of Missouri, Columbia, Missouri, USA aut NATIONALLICENCE 700 1- Fent Genevieve Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA aut NATIONALLICENCE 700 1- Casteel Stan Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA aut NATIONALLICENCE 700 1- Boote Evan Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut NATIONALLICENCE 700 1- Viator John Department of Biological Engineering, University of Missouri, Columbia, Missouri, USA aut NATIONALLICENCE 700 1- Upendran Anandhi Nanoparticle Biochem, Inc., 65201, Columbia, Missouri, USA aut NATIONALLICENCE 700 1- Kannan Raghuraman Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut NATIONALLICENCE 700 1- Katti Kattesh Department of Radiology, University of Missouri, 301 Business Loop 70W, Columbia, Missouri, USA aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/2(2011-02-01), 279-291 0724-8741 28:2<279 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer