caa a22 4500 445314125 CHVBK 20180317142640.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s11095-010-0279-3 doi (NATIONALLICENCE)springer-10.1007/s11095-010-0279-3 Evidence of Deep Percutaneous Penetration Associated with Anti-Inflammatory Activity of Topically Applied Helicteres gardneriana Extract: A Photoacoustic Spectroscopy Study [Elektronische Daten] [Juliana Oliveira de Melo, Franciana Pedrochi, Mauro Baesso, Luzmarina Hernandes, Maria Truiti, Silmara Baroni, Ciomar Bersani-Amado] ABSTRACT: Purpose: To apply the photoacoustic spectroscopy technique to investigate the penetration rate of topically applied Helicteres gardneriana extract used as anti-inflammatory agent. Methods: Experiments were performed ex vivo in a well-controlled group of mice. The crude extract was obtained from leaves of the plant Helicteres gardneriana. Croton oil was applied into the ventral surface of the mouse's right and left auricles in order to induce an inflammatory response. The left auricle was treated with crude extract, while the right one served as the control. After 6h, the auricles were sectioned for measurements of edema intensity, myeloperoxidase activity and the formulation penetration rate. Results: Croton oil induced inflammatory response in both auricles. The application of Helicteres gardneriana extract reduced significantly the edema of the auricle and inhibited the activity of the myeloperoxidase enzyme. The photoacoustic data showed that the propagation of the formulation was efficient to reach the deep region of the auricle, crossing the cartilage. The strong anti-inflammatory effect was associated with the observed deep penetration of the formulation. Conclusion: This pre-clinical study showed the anti-inflammatory effect of Helicteres gardneriana extract. The photoacoustic technique was useful to demonstrate that this anti-inflammatory activity was associated with deep percutaneous penetration. Springer Science+Business Media, LLC, 2010 anti-inflammatory agent nationallicence Helicteres gardneriana extract nationallicence percutaneous penetration nationallicence photoacoustic spectroscopy nationallicence Oliveira de Melo Juliana Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil aut Pedrochi Franciana Departamento de Física, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil aut Baesso Mauro Departamento de Física, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil aut Hernandes Luzmarina Departamento de Ciências Morfológicas, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil aut Truiti Maria Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil aut Baroni Silmara Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil aut Bersani-Amado Ciomar Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/2(2011-02-01), 331-336 0724-8741 28:2<331 2011 28 11095 https://doi.org/10.1007/s11095-010-0279-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-010-0279-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oliveira de Melo Juliana Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil aut NATIONALLICENCE 700 1- Pedrochi Franciana Departamento de Física, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil aut NATIONALLICENCE 700 1- Baesso Mauro Departamento de Física, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil aut NATIONALLICENCE 700 1- Hernandes Luzmarina Departamento de Ciências Morfológicas, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil aut NATIONALLICENCE 700 1- Truiti Maria Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil aut NATIONALLICENCE 700 1- Baroni Silmara Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil aut NATIONALLICENCE 700 1- Bersani-Amado Ciomar Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá, Pr, Brazil aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/2(2011-02-01), 331-336 0724-8741 28:2<331 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer