caa a22 4500 445314257 CHVBK 20180317142640.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s11095-011-0544-0 doi (NATIONALLICENCE)springer-10.1007/s11095-011-0544-0 Expression of GFP in the Mitochondrial Compartment Using DQAsome-Mediated Delivery of an Artificial Mini-mitochondrial Genome [Elektronische Daten] [Diana Lyrawati, Alan Trounson, David Cram] ABSTRACT: Purpose: We describe a novel strategy for expression of GFP in mammalian mitochondria. Methods: The key components of the strategy were an artificially created mitochondrial genome pmtGFP and a DQAsome transfection system. Results: Using immunofluorescence and a combination of immunohistochemical and molecular based techniques, we show that DQAsomes are capable of delivering the pmtGFP construct to the mitochondrial compartment of the mouse macrophage cell line RAW264.7, albeit at low efficiency (1-5%), resulting in the expression of GFP mRNA and protein. Similar transfection efficiencies were also demonstrated in a range of other mammalian cell lines. Conclusions: The DQAsome-transfection technique was able to deliver the exogenous DNA into the cellular mitochondria and the pmtGFP was functional. Further optimization of this strategy would provide a flexible and rapid way to generate mutant cells and useful animal models of mitochondrial disease. Springer Science+Business Media, LLC, 2011 DQAsome nationallicence GFP expression nationallicence mini-mitochondrial genome nationallicence mitochondrial gene delivery nationallicence transfection nationallicence Lyrawati Diana Laboratory of Pharmacy, Faculty of Medicine, Brawijaya University, Jl. Veteran, Malang, Indonesia aut Trounson Alan California Institute for Regenerative Medicine, 94107, San Francisco, California, USA aut Cram David Monash Immunology and Stem Cell Laboratories, Monash University, Melbourne, Australia aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/11(2011-11-01), 2848-2862 0724-8741 28:11<2848 2011 28 11095 https://doi.org/10.1007/s11095-011-0544-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-011-0544-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lyrawati Diana Laboratory of Pharmacy, Faculty of Medicine, Brawijaya University, Jl. Veteran, Malang, Indonesia aut NATIONALLICENCE 700 1- Trounson Alan California Institute for Regenerative Medicine, 94107, San Francisco, California, USA aut NATIONALLICENCE 700 1- Cram David Monash Immunology and Stem Cell Laboratories, Monash University, Melbourne, Australia aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/11(2011-11-01), 2848-2862 0724-8741 28:11<2848 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer