caa a22 4500 445314281 CHVBK 20180317142640.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s11095-011-0531-5 doi (NATIONALLICENCE)springer-10.1007/s11095-011-0531-5 The Utility of an Isolated Mitochondrial Fraction in the Preparation of Liposomes for the Specific Delivery of Bioactives to Mitochondria in Live Mammalian Cells [Elektronische Daten] [Mayura Wagle, Laura Martinville, Gerard D'Souza] ABSTRACT: Purpose: To develop and evaluate liposomal formulations prepared with an isolated mitochondrial fraction as a mitochondria-specific delivery vehicle Methods: Liposomes were prepared with either a crude mitochondrial fraction extracted from cells or lipids extracted from the crude mitochondrial fraction and were then characterized by determining their size and zeta potential. The cell uptake of the liposomes and loaded bioactive was studied using flow cytometry and confocal microscopy. The cytotoxicity of the formulations was tested by MTS cytotoxicity assay. Results: Liposomes prepared with the mitochondrial extracts were non-toxic and colocalized with mitochondria in F98 cells. Addition of DOTAP to the liposomes facilitated DNA complexation and the DNA delivered intracellularly co-localized with mitochondria. Conclusion: The results from this study establish the potential of using a mitochondrial fraction isolated from cells to prepare liposomes capable of delivering biologically active molecules to mitochondria of live mammalian cells. Springer Science+Business Media, LLC, 2011 DNA delivery nationallicence liposomes nationallicence mitochondria nationallicence sub-cellular nationallicence targeting nationallicence Wagle Mayura Department of Pharmaceutical Sciences, Massachusetts College of Pharmacy and Health Sciences, 179 Longwood Avenue, 02115, Boston, Massachusetts, USA aut Martinville Laura Department of Pharmaceutical Sciences, Massachusetts College of Pharmacy and Health Sciences, 179 Longwood Avenue, 02115, Boston, Massachusetts, USA aut D'Souza Gerard Department of Pharmaceutical Sciences, Massachusetts College of Pharmacy and Health Sciences, 179 Longwood Avenue, 02115, Boston, Massachusetts, USA aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/11(2011-11-01), 2790-2796 0724-8741 28:11<2790 2011 28 11095 https://doi.org/10.1007/s11095-011-0531-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-011-0531-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wagle Mayura Department of Pharmaceutical Sciences, Massachusetts College of Pharmacy and Health Sciences, 179 Longwood Avenue, 02115, Boston, Massachusetts, USA aut NATIONALLICENCE 700 1- Martinville Laura Department of Pharmaceutical Sciences, Massachusetts College of Pharmacy and Health Sciences, 179 Longwood Avenue, 02115, Boston, Massachusetts, USA aut NATIONALLICENCE 700 1- D'Souza Gerard Department of Pharmaceutical Sciences, Massachusetts College of Pharmacy and Health Sciences, 179 Longwood Avenue, 02115, Boston, Massachusetts, USA aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/11(2011-11-01), 2790-2796 0724-8741 28:11<2790 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer