caa a22 4500 445314370 CHVBK 20180317142641.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s11095-011-0492-8 doi (NATIONALLICENCE)springer-10.1007/s11095-011-0492-8 Quantitative Glucose and ATP Sensing in Mammalian Cells [Elektronische Daten] [Dania Liemburg-Apers, Hiromi Imamura, Marleen Forkink, Marco Nooteboom, Herman Swarts, Roland Brock, Jan Smeitink, Peter Willems, Werner Koopman] ABSTRACT: The functioning and survival of mammalian cells requires an active energy metabolism. Metabolic dysfunction plays an important role in many human diseases, including diabetes, cancer, inherited mitochondrial disorders, and metabolic syndrome. The monosaccharide glucose constitutes a key source of cellular energy. Following its import across the plasma membrane, glucose is converted into pyruvate by the glycolysis pathway. Pyruvate oxidation supplies substrates for the ATP-generating mitochondrial oxidative phosphorylation (OXPHOS) system. To gain cell-biochemical knowledge about the operation and regulation of the cellular energy metabolism in the healthy and diseased state, quantitative knowledge is required about (changes in) metabolite concentrations under (non) steady-state conditions. This information can, for instance, be used to construct more realistic in silico models of cell metabolism, which facilitates understanding the consequences of metabolic dysfunction as well as on- and off-target effects of mitochondrial drugs. Here we review the current state-of-the-art live-cell quantification of two key cellular metabolites, glucose and ATP, using protein-based sensors. The latter apply the principle of FRET (fluorescence resonance energy transfer) and allow measurements in different cell compartments by fluorescence microscopy. We further summarize the properties and applications of the FRET-based sensors, their calibration, pitfalls, and future perspectives. Springer Science+Business Media, LLC, 2011 ATeam nationallicence fibroblast nationallicence GLUT nationallicence systems biology nationallicence 2-DG : 2-Deoxy-D-glucose nationallicence A : fluorescence acceptor molecule nationallicence AcCoA : acetyl-coenzyme A nationallicence ADP : adenoside diphosphate nationallicence AMP : adenosine monophosphate nationallicence AMPK : AMP-activated protein kinase nationallicence ANT : adenine nucleotide translocator nationallicence ATP : adenoside triphosphate nationallicence D : fluorescence donor molecule nationallicence DNP : 2,4-Dinitrophenol nationallicence ECFP : enhanced cyan fluorescent protein nationallicence ER : endoplasmic reticulum nationallicence EYFP : enhanced yellow fluorescent protein nationallicence FCCP : carbonyl cyanide-p-trifluoromethoxyphenylhydrazone nationallicence FRET : fluorescence resonance energy transfer nationallicence FS : fractional saturation nationallicence G6P : glucose-6-phosphate nationallicence GFP : green fluorescent protein nationallicence GGBP : glucose galactose-binding protein nationallicence GK : glucokinase nationallicence GLUT : glucose transporter nationallicence HK : hexokinase nationallicence IAA : iodoacetate nationallicence LDH : lactate dehydrogenase nationallicence OFP : orange fluorescent protein nationallicence OXPHOS : oxidative phosphorylation nationallicence PBP : periplasmic binding protein nationallicence PDH : pyruvate dehydrogenase nationallicence PFK : phosphofructokinase nationallicence PK : pyruvate kinase nationallicence PKC : protein kinase C nationallicence PM : plasma membrane nationallicence PPP : pentose phosphate pathway nationallicence ROS : reactive oxygen species nationallicence SGLT : sodium-dependent glucose cotransporters nationallicence SLO : streptolysin O nationallicence SNR : signal-to-noise ratio nationallicence TCA : tricarboxylic acid nationallicence TPA : phorbol 12-myristate 13-acetate nationallicence VDAC : voltage-dependent anion channel nationallicence Liemburg-Apers Dania Department of Biochemistry (286) Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands aut Imamura Hiromi Kyoto University, Kyoto, Japan aut Forkink Marleen Department of Biochemistry (286) Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands aut Nooteboom Marco Department of Biochemistry (286) Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands aut Swarts Herman Department of Biochemistry (286) Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands aut Brock Roland Department of Biochemistry (286) Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands aut Smeitink Jan Department of Pediatrics Nijmegen Centre for Mitochondrial Disorders, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands aut Willems Peter Department of Biochemistry (286) Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands aut Koopman Werner Department of Biochemistry (286) Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/11(2011-11-01), 2745-2757 0724-8741 28:11<2745 2011 28 11095 https://doi.org/10.1007/s11095-011-0492-8 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-011-0492-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liemburg-Apers Dania Department of Biochemistry (286) Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Imamura Hiromi Kyoto University, Kyoto, Japan aut NATIONALLICENCE 700 1- Forkink Marleen Department of Biochemistry (286) Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Nooteboom Marco Department of Biochemistry (286) Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Swarts Herman Department of Biochemistry (286) Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Brock Roland Department of Biochemistry (286) Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Smeitink Jan Department of Pediatrics Nijmegen Centre for Mitochondrial Disorders, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Willems Peter Department of Biochemistry (286) Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Koopman Werner Department of Biochemistry (286) Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/11(2011-11-01), 2745-2757 0724-8741 28:11<2745 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer