caa a22 4500 445314478 CHVBK 20180317142641.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s11095-011-0515-5 doi (NATIONALLICENCE)springer-10.1007/s11095-011-0515-5 Time-Correlated Single Photon Counting For Simultaneous Monitoring Of Zinc Oxide Nanoparticles And NAD(P)H In Intact And Barrier-Disrupted Volunteer Skin [Elektronische Daten] [Lynlee Lin, Jeffrey Grice, Margaret Butler, Andrei Zvyagin, Wolfgang Becker, Thomas Robertson, H. Soyer, Michael Roberts, Tarl Prow] ABSTRACT: Purpose: There is a lack of relevant, non-animal alternatives for assessing exposure and toxicity of nanoparticle-containing cosmetics, e.g. sunscreens. Our goal was to evaluate timecorrelated single photon counting (TCSPC) for simultaneous monitoring of zinc oxide nanoparticles (ZnO-NP) and the metabolic state of volunteer skin. Methods: We separated the fluorescence lifetime signatures of endogenous fluorophore signals (i.e. nicotinamide adenine dinucleotide phosphate, NAD(P)H and keratin) and the ZnO-NP signal using advanced TCSPC to simultaneously determine ZnO-NP penetration profiles and NAD(P)H changes in subjects with altered barrier function, including tape-stripped skin and in psoriasis or atopic dermatitis lesions. Results: We detected no ZnO-NP penetration into viable human skin in any group. ZnO-NP signal was significantly increased (p < 0.01) on the surface of tape-stripped and lesional skin after 4 and 2h of treatment, respectively. Free NAD(P)H signal significantly increased in tape-stripped viable epidermis treated for 4h of ZnO-NP compared to vehicle control. No significant NAD(P)H changes were noted in the lesional study. Conclusion: TCSPC techniques enabled simultaneous, real-time quantification of ZnO-NP concentration and NAD(P)H via non-invasive imaging in the stratum corneum and viable epidermis of volunteers. Springer Science+Business Media, LLC, 2011 human skin nationallicence metabolism nationallicence multiphoton microscopy nationallicence sunscreen nationallicence zinc oxide nanoparticle nationallicence AAS : atomic absorption spectroscopy nationallicence AU : arbitrary unit nationallicence CCT : caprylic/capric triglycerides nationallicence FLIM : fluorescence lifetime imaging microscopy nationallicence ICP-OES : inductively coupled plasma-optical emission spectroscopy nationallicence IRF : instrument response function nationallicence KDP : potassium di-hydrogen phosphate nationallicence MEP : multiphoton-excited photoluminescence nationallicence MPT : multiphoton tomography nationallicence MPT-FLIM : multiphoton tomography with fluorescence lifetime imaging microscopy nationallicence NAD(P)H : nicotinamide adenine dinucleotide phosphate nationallicence PBS : phosphate-buffered saline nationallicence SHG : second harmonic generation nationallicence TCSPC : time-correlated single photon counting nationallicence TEM : transmission electron microscope nationallicence TEWL : transepidermal Water Loss nationallicence Ti:Sa : titanium:sapphire nationallicence ZnO-NP : zinc oxide nanoparticles nationallicence Lin Lynlee Therapeutics Research Centre, School of Medicine Princess Alexandra Hospital, University of Queensland, Brisbane, Queensland, Australia aut Grice Jeffrey Therapeutics Research Centre, School of Medicine Princess Alexandra Hospital, University of Queensland, Brisbane, Queensland, Australia aut Butler Margaret Australian Institute for Bioengineering & Nanotechnology, University of Queensland, Brisbane, Queensland, Australia aut Zvyagin Andrei Department of Physics, Centre of MQ Photonics, Macquarie University, Sydney, New South Wales, Australia aut Becker Wolfgang Becker & Hickl GmbH, Nahmitzer Damm 33, Berlin, Germany aut Robertson Thomas Therapeutics Research Centre School of Pharmacy & Biomedical Sciences, University of South Australia, Adelaide, South Australia, Australia aut Soyer H. Dermatology Research Centre, School of Medicine Princess Alexandra Hospital, University of Queensland, Brisbane, Queensland, Australia aut Roberts Michael Therapeutics Research Centre, School of Medicine Princess Alexandra Hospital, University of Queensland, Brisbane, Queensland, Australia aut Prow Tarl Therapeutics Research Centre, School of Medicine Princess Alexandra Hospital, University of Queensland, Brisbane, Queensland, Australia aut Pharmaceutical Research Springer US; http://www.springer-ny.com 28/11(2011-11-01), 2920-2930 0724-8741 28:11<2920 2011 28 11095 https://doi.org/10.1007/s11095-011-0515-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11095-011-0515-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lin Lynlee Therapeutics Research Centre, School of Medicine Princess Alexandra Hospital, University of Queensland, Brisbane, Queensland, Australia aut NATIONALLICENCE 700 1- Grice Jeffrey Therapeutics Research Centre, School of Medicine Princess Alexandra Hospital, University of Queensland, Brisbane, Queensland, Australia aut NATIONALLICENCE 700 1- Butler Margaret Australian Institute for Bioengineering & Nanotechnology, University of Queensland, Brisbane, Queensland, Australia aut NATIONALLICENCE 700 1- Zvyagin Andrei Department of Physics, Centre of MQ Photonics, Macquarie University, Sydney, New South Wales, Australia aut NATIONALLICENCE 700 1- Becker Wolfgang Becker & Hickl GmbH, Nahmitzer Damm 33, Berlin, Germany aut NATIONALLICENCE 700 1- Robertson Thomas Therapeutics Research Centre School of Pharmacy & Biomedical Sciences, University of South Australia, Adelaide, South Australia, Australia aut NATIONALLICENCE 700 1- Soyer H. Dermatology Research Centre, School of Medicine Princess Alexandra Hospital, University of Queensland, Brisbane, Queensland, Australia aut NATIONALLICENCE 700 1- Roberts Michael Therapeutics Research Centre, School of Medicine Princess Alexandra Hospital, University of Queensland, Brisbane, Queensland, Australia aut NATIONALLICENCE 700 1- Prow Tarl Therapeutics Research Centre, School of Medicine Princess Alexandra Hospital, University of Queensland, Brisbane, Queensland, Australia aut NATIONALLICENCE 773 0- Pharmaceutical Research Springer US; http://www.springer-ny.com 28/11(2011-11-01), 2920-2930 0724-8741 28:11<2920 2011 28 11095 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer