caa a22 4500 445314788 CHVBK 20180317142642.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10552-011-9757-0 doi (NATIONALLICENCE)springer-10.1007/s10552-011-9757-0 Diagnostic and prognostic validity of the human papillomavirus E6/E7 mRNA test in cervical cytological samples of HC2-positive patients [Elektronische Daten] [Maria Benevolo, Irene Terrenato, Marcella Mottolese, Ferdinando Marandino, Mariantonia Carosi, Francesca Rollo, Livia Ronchetti, Paola Muti, Luciano Mariani, Stefano Sindico, Giuseppe Vocaturo, Amina Vocaturo] The study aimed to assess the clinical utility in identifying CIN2 or worse (CIN2+), of the Pretect HPV-Proofer test for E6/E7 mRNA detection in Hybrid Capture 2 (HC2)-positive patients, who underwent colposcopy. In particular, the study analyzed the mRNA test performance as the third test in a subgroup of HC2+ patients with less severe than high-grade squamous intraepithelial lesions (HSIL−). We analyzed 464 cervico-vaginal samples by liquid-based cytology (LBC) and PreTect HPV-Proofer. Moreover 231 patients also had a biopsy at baseline and 75, with HSIL−, were followed up within 2years by LBC, colposcopy, and histology when indicated. The highest sensitivity for CIN2+ belonged to the mRNA compared to LBC, at the HSIL+ threshold (72% vs. 58%), whereas the LBC showed the highest specificity and positive predictive value (PPV) (99 and 93% vs. 73 and 39%, respectively). Focusing on the 408 HSIL− patients, the mRNA positivity was significantly more associated with CIN2+ than CIN2− lesions (p<0.0001). Moreover, among the 75 HSIL− followed up patients, the mRNA displayed high longitudinal Specificity (89%), even if the sensitivity and the PPV were low (50 and 20%, respectively). The present data suggest that the mRNA test may have a diagnostic and a potentially prognostic role in HC2+/HSIL− patients. Springer Science+Business Media B.V., 2011 Cervical lesions nationallicence Diagnostic biomarker nationallicence HPV E6/E7 mRNA nationallicence Human papillomavirus nationallicence Prognostic biomarker nationallicence Benevolo Maria Pathology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut Terrenato Irene Epidemiology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut Mottolese Marcella Pathology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut Marandino Ferdinando Pathology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut Carosi Mariantonia Pathology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut Rollo Francesca Pathology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut Ronchetti Livia Pathology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut Muti Paola Scientific Direction, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut Mariani Luciano Oncologic Gynecology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut Sindico Stefano Oncologic Gynecology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut Vocaturo Giuseppe Oncologic Gynecology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut Vocaturo Amina Pathology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut Cancer Causes & Control Springer Netherlands 22/6(2011-06-01), 869-875 0957-5243 22:6<869 2011 22 10552 https://doi.org/10.1007/s10552-011-9757-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10552-011-9757-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Benevolo Maria Pathology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut NATIONALLICENCE 700 1- Terrenato Irene Epidemiology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut NATIONALLICENCE 700 1- Mottolese Marcella Pathology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut NATIONALLICENCE 700 1- Marandino Ferdinando Pathology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut NATIONALLICENCE 700 1- Carosi Mariantonia Pathology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut NATIONALLICENCE 700 1- Rollo Francesca Pathology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut NATIONALLICENCE 700 1- Ronchetti Livia Pathology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut NATIONALLICENCE 700 1- Muti Paola Scientific Direction, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut NATIONALLICENCE 700 1- Mariani Luciano Oncologic Gynecology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut NATIONALLICENCE 700 1- Sindico Stefano Oncologic Gynecology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut NATIONALLICENCE 700 1- Vocaturo Giuseppe Oncologic Gynecology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut NATIONALLICENCE 700 1- Vocaturo Amina Pathology Department, Regina Elena Cancer Institute, Via Elio Chianesi 53, 00144, Rome, Italy aut NATIONALLICENCE 773 0- Cancer Causes & Control Springer Netherlands 22/6(2011-06-01), 869-875 0957-5243 22:6<869 2011 22 10552 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer