caa a22 4500 445318023 CHVBK 20180317142653.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10126-010-9267-1 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9267-1 Can the Quality of Pearls from the Japanese Pearl Oyster ( Pinctada fucata ) be Explained by the Gene Expression Patterns of the Major Shell Matrix Proteins in the Pearl Sac? [Elektronische Daten] [Nariaki Inoue, Ryo Ishibashi, Takashi Ishikawa, Takashi Atsumi, Hideo Aoki, Akira Komaru] For pearl culture, the pearl oyster is forced open and a nucleus is implanted into the gonad with a mantle graft. The outer mantle epithelial cells of the implanted mantle graft elongate and surrounding the nucleus a pearl sac is formed. Shell matrix proteins secreted by the pearl sac play an important role in the regulation of pearl formation. Recently, seven shell matrix proteins were identified from the pearl oyster Pinctada fucata. However, there is a paucity of information on the function of these proteins and their gene expression patterns. Our study aims to elucidate the relationship between pearl type, quality, and gene expression patterns of six shell matrix proteins (msi60, n16, nacrein, msi31, prismalin-14, and aspein) in the pearl sac based on real-time PCR analysis. After culturing for about 2months, the pearl sac tissues were collected from 22 individuals: 12 with high quality (HP), nine with low quality (LP), and one with organic (ORG) pearl formation. The surface of each of the 12 HP pearls was composed only of a nacreous layer; in contrast, that of the nine LP pearls was composed of nacreous and prismatic layers. The six target gene expressions were detected in all individuals. However, delta threshold cycle (ΔC T) for msi31 was significantly higher in the HP than in the LP individuals (Mann-Whitney's U test, p = 0.02). This means that the relative expression level of msi31, which constitutes the framework of the prismatic layer, was higher in the LP than in the HP individuals. Springer Science+Business Media, LLC, 2010 Pearl nationallicence Pinctada fucata nationallicence Prismatic layer nationallicence Shell matrix proteins nationallicence Real-time PCR nationallicence Gene expression nationallicence Inoue Nariaki Faculty of Bioresources, Mie University, 1577 Kurimamachiya, 514-0501, Tsu, Mie, Japan aut Ishibashi Ryo Faculty of Bioresources, Mie University, 1577 Kurimamachiya, 514-0501, Tsu, Mie, Japan aut Ishikawa Takashi Faculty of Bioresources, Mie University, 1577 Kurimamachiya, 514-0501, Tsu, Mie, Japan aut Atsumi Takashi Mie Prefecture Fisheries Research Institute, 3564-3 Hamajima, 517-0404, Shima, Mie, Japan aut Aoki Hideo Mie Prefecture Fisheries Research Institute, 3564-3 Hamajima, 517-0404, Shima, Mie, Japan aut Komaru Akira Faculty of Bioresources, Mie University, 1577 Kurimamachiya, 514-0501, Tsu, Mie, Japan aut Marine Biotechnology Springer-Verlag 13/1(2011-02-01), 48-55 1436-2228 13:1<48 2011 13 10126 https://doi.org/10.1007/s10126-010-9267-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9267-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Inoue Nariaki Faculty of Bioresources, Mie University, 1577 Kurimamachiya, 514-0501, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Ishibashi Ryo Faculty of Bioresources, Mie University, 1577 Kurimamachiya, 514-0501, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Ishikawa Takashi Faculty of Bioresources, Mie University, 1577 Kurimamachiya, 514-0501, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Atsumi Takashi Mie Prefecture Fisheries Research Institute, 3564-3 Hamajima, 517-0404, Shima, Mie, Japan aut NATIONALLICENCE 700 1- Aoki Hideo Mie Prefecture Fisheries Research Institute, 3564-3 Hamajima, 517-0404, Shima, Mie, Japan aut NATIONALLICENCE 700 1- Komaru Akira Faculty of Bioresources, Mie University, 1577 Kurimamachiya, 514-0501, Tsu, Mie, Japan aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/1(2011-02-01), 48-55 1436-2228 13:1<48 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer