caa a22 4500 44531804X CHVBK 20180317142653.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10126-010-9266-2 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9266-2 Ploidy Manipulation and Polyploid Detection in the White Shrimp Litopenaeus vannamei (Boone 1931) (Decapoda, Penaeidae) [Elektronische Daten] [Débora de Almeida Aloise, Francisco de Assis Maia-Lima, Ruth de Oliveira, Thiago de Melo Cabral, Wagner Molina] Ploidy manipulation has been rarely used in the genetic improvement of cultured marine shrimps. Although polyploid induction has been proven to be successful in Penaeids, including the species Litopenaeus vannamei, the methodology still requires some improvements. In the present work, different thermal shock treatments on ploidy manipulation were tested and a protocol for detecting polyploid individuals was also established. Fertilized eggs were treated by cold (10°C) and heat (38°C) thermal shocks for 8, 12, 15, 18, 20, and 22min to induce polyploidy. Nuclear measurements within distinct treatments revealed a significant deviation in relation to the mean diameter of nuclei in the control individuals. Triploid and tetraploid metaphases were observed within treated individuals, confirming the increase of interphasic nuclear diameter. The cold thermal shock was more efficient than the hot ones, besides leading to a higher and more homogeneous hatchery rate. A mean number of three nucleoli per nucleus were observed in diploid individuals, while treated samples usually presented up to five nucleoli per nucleus. The standardization of protocols to obtain and detect polyploid products allows further utilization of such methods on a commercial scale in order to evaluate the performance of polyploid individuals in the genetic improvement of L. vannamei. Springer Science+Business Media, LLC, 2010 Polyploidy nationallicence Crustacea nationallicence Litopenaeus vannamei nationallicence Crustacean cytogenetics nationallicence Ag-NORs nationallicence de Almeida Aloise Débora Departamento de Biologia Celular e Genética, Centro de Biociências Universidade Federal do Rio Grande do Norte, 59078-970, Natal, Rio Grande do Norte, Brazil aut de Assis Maia-Lima Francisco Departamento de Biologia, Faculdade de Ciências, Cultura e Extensão do Rio Grande do Norte-FACEX, 59080-020, Natal, Rio Grande do Norte, Brazil aut de Oliveira Ruth Departamento de Biologia Celular e Genética, Centro de Biociências Universidade Federal do Rio Grande do Norte, 59078-970, Natal, Rio Grande do Norte, Brazil aut de Melo Cabral Thiago Departamento de Biologia Celular e Genética, Centro de Biociências Universidade Federal do Rio Grande do Norte, 59078-970, Natal, Rio Grande do Norte, Brazil aut Molina Wagner Departamento de Biologia Celular e Genética, Centro de Biociências Universidade Federal do Rio Grande do Norte, 59078-970, Natal, Rio Grande do Norte, Brazil aut Marine Biotechnology Springer-Verlag 13/1(2011-02-01), 41-47 1436-2228 13:1<41 2011 13 10126 https://doi.org/10.1007/s10126-010-9266-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9266-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Almeida Aloise Débora Departamento de Biologia Celular e Genética, Centro de Biociências Universidade Federal do Rio Grande do Norte, 59078-970, Natal, Rio Grande do Norte, Brazil aut NATIONALLICENCE 700 1- de Assis Maia-Lima Francisco Departamento de Biologia, Faculdade de Ciências, Cultura e Extensão do Rio Grande do Norte-FACEX, 59080-020, Natal, Rio Grande do Norte, Brazil aut NATIONALLICENCE 700 1- de Oliveira Ruth Departamento de Biologia Celular e Genética, Centro de Biociências Universidade Federal do Rio Grande do Norte, 59078-970, Natal, Rio Grande do Norte, Brazil aut NATIONALLICENCE 700 1- de Melo Cabral Thiago Departamento de Biologia Celular e Genética, Centro de Biociências Universidade Federal do Rio Grande do Norte, 59078-970, Natal, Rio Grande do Norte, Brazil aut NATIONALLICENCE 700 1- Molina Wagner Departamento de Biologia Celular e Genética, Centro de Biociências Universidade Federal do Rio Grande do Norte, 59078-970, Natal, Rio Grande do Norte, Brazil aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/1(2011-02-01), 41-47 1436-2228 13:1<41 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer