caa a22 4500 445318082 CHVBK 20180317142654.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10126-010-9270-6 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9270-6 Enzymatic Extract from Ecklonia cava Induces the Activation of Lymphocytes by IL-2 Production Through the Classical NF-κB Pathway [Elektronische Daten] [Ginnae Ahn, Eunjin Park, Won-Woo Lee, Jin-Won Hyun, Ki-Wan Lee, Taekyun Shin, You-Jin Jeon, Youngheun Jee] Activated nuclear factor-kappa B (NF-κB), a well-known transcription factor, leads to the development, differentiation, and proliferation of T and B lymphocytes and the secretion of cytokines by the classical pathway. We have examined here whether an enzymatic extract (ECK) from the brown seaweed, Alariaceae Laminariales Ecklonia cava may contribute to activating lymphocytes through the NF-κB pathway for participation in immune responses. In our study, ECK dose-dependently enhanced the proliferation of lymphocytes. ECK significantly increased the phosphorylation of inhibitors of κB at 0.25 and 0.5h of exposure, followed by its gradual decrease. In addition, NF-κB p65 was gradually activated, and its binding to nuclear deoxyribonucleic acid was observed from 0.25h after stimulation (up to 0.5h). Further experiments showed that the application of N-p-tosyl-l-phenylalanine chloromethyl ketone, an NF-κB inhibitor, significantly blocked ECK-induced lymphocyte's proliferation and the interleukin (IL)-2 productions. Accordingly, our results suggest that ECK increases the production of IL-2 through the activation of NF-κB then induces the proliferation of lymphocytes with the coordinated stimulation of IL-2. Springer Science+Business Media, LLC, 2010 Ecklonia cava nationallicence Lymphocyte nationallicence NF-κB nationallicence IL-2 nationallicence Ahn Ginnae Department of Marine Life Science, Jeju National University, 690-756, Jeju, Republic of Korea aut Park Eunjin College of Veterinary Medicine and Applied Radiological Science Institute, Jeju National University, 690-756, Jeju, Republic of Korea aut Lee Won-Woo Department of Marine Life Science, Jeju National University, 690-756, Jeju, Republic of Korea aut Hyun Jin-Won Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Jeju National University, 690-756, Jeju, Republic of Korea aut Lee Ki-Wan Department of Marine Life Science, Jeju National University, 690-756, Jeju, Republic of Korea aut Shin Taekyun College of Veterinary Medicine and Applied Radiological Science Institute, Jeju National University, 690-756, Jeju, Republic of Korea aut Jeon You-Jin Department of Marine Life Science, Jeju National University, 690-756, Jeju, Republic of Korea aut Jee Youngheun College of Veterinary Medicine and Applied Radiological Science Institute, Jeju National University, 690-756, Jeju, Republic of Korea aut Marine Biotechnology Springer-Verlag 13/1(2011-02-01), 66-73 1436-2228 13:1<66 2011 13 10126 https://doi.org/10.1007/s10126-010-9270-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9270-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ahn Ginnae Department of Marine Life Science, Jeju National University, 690-756, Jeju, Republic of Korea aut NATIONALLICENCE 700 1- Park Eunjin College of Veterinary Medicine and Applied Radiological Science Institute, Jeju National University, 690-756, Jeju, Republic of Korea aut NATIONALLICENCE 700 1- Lee Won-Woo Department of Marine Life Science, Jeju National University, 690-756, Jeju, Republic of Korea aut NATIONALLICENCE 700 1- Hyun Jin-Won Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Jeju National University, 690-756, Jeju, Republic of Korea aut NATIONALLICENCE 700 1- Lee Ki-Wan Department of Marine Life Science, Jeju National University, 690-756, Jeju, Republic of Korea aut NATIONALLICENCE 700 1- Shin Taekyun College of Veterinary Medicine and Applied Radiological Science Institute, Jeju National University, 690-756, Jeju, Republic of Korea aut NATIONALLICENCE 700 1- Jeon You-Jin Department of Marine Life Science, Jeju National University, 690-756, Jeju, Republic of Korea aut NATIONALLICENCE 700 1- Jee Youngheun College of Veterinary Medicine and Applied Radiological Science Institute, Jeju National University, 690-756, Jeju, Republic of Korea aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/1(2011-02-01), 66-73 1436-2228 13:1<66 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer