caa a22 4500 445318139 CHVBK 20180317142654.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10126-011-9374-7 doi (NATIONALLICENCE)springer-10.1007/s10126-011-9374-7 Gene Expression Profiles of the Spleen, Liver, and Head Kidney in Turbot ( Scophthalmus maximus ) Along the Infection Process with Aeromonas salmonicida Using an Immune-Enriched Oligo-microarray [Elektronische Daten] [Adrián Millán, Antonio Gómez-Tato, Belén Pardo, Carlos Fernández, Carmen Bouza, Manuel Vera, José Alvarez-Dios, Santiago Cabaleiro, Jesús Lamas, Manuel Lemos, Paulino Martínez] We evaluated the expression profiles of turbot in the spleen, liver, and head kidney across five temporal points of the Aeromonas salmonicida infection process using an 8 × 15K Agilent oligo-microarray. The microarray included 2,176 different fivefold replicated gene probes designed from a turbot 3′ sequenced EST database. We were able to identify 471 differentially expressed (DE) genes (17.3% of the whole microarray), 223 in the spleen, 246 in the liver, and 125 in the head kidney, in at least one of the five temporal points sampled for each organ. Most of these genes could be annotated (83.0%) and functionally categorized using Gene Ontology terms (69.1%) after the additional sequencing of DE genes from the 5′ end. Many DE genes were related to innate and acquired immune functions in accordance to previous studies with this pathogen in other fish species. A high proportion of DE genes were organ specific (77.1%), but their associated GO functions were rather similar in the three organs. The most striking difference in functional distribution was observed between the up- and down-regulated gene groups. Up-regulated genes were mostly associated to key immune functions while down-regulated ones mainly involved metabolism- and transport-related genes. Genetic response appeared clustered in groups of genes with similar expression profiles along the temporal series. The spleen showed the most clustering while the liver and head kidney displayed a higher diversification. The information obtained will aid to understand the turbot immune response and will specifically be valuable to develop strategies of defense to A. salmonicida to achieve more resistant broodstocks for turbot industry. Springer Science+Business Media, LLC, 2011 Turbot nationallicence Scophthalmus maximus nationallicence Aeromonas salmonicida nationallicence Oligo-microarray nationallicence Immune response nationallicence Functional classification nationallicence Millán Adrián Departamento de Genética, Facultad de Veterinaria, Campus de Lugo, Universidad de Santiago de Compostela, 27002, Lugo, Spain aut Gómez-Tato Antonio Departamento de Geometría y Topología, Facultad de Matemáticas, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut Pardo Belén Departamento de Genética, Facultad de Veterinaria, Campus de Lugo, Universidad de Santiago de Compostela, 27002, Lugo, Spain aut Fernández Carlos Departamento de Genética, Facultad de Veterinaria, Campus de Lugo, Universidad de Santiago de Compostela, 27002, Lugo, Spain aut Bouza Carmen Departamento de Genética, Facultad de Veterinaria, Campus de Lugo, Universidad de Santiago de Compostela, 27002, Lugo, Spain aut Vera Manuel Departamento de Genética, Facultad de Veterinaria, Campus de Lugo, Universidad de Santiago de Compostela, 27002, Lugo, Spain aut Alvarez-Dios José Departamento de Matemática Aplicada, Facultad de Matemáticas, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut Cabaleiro Santiago Cluster de la Acuicultura de Galicia (CETGA), Punta de Couso s/n, Aguiño, 15965, A Coruña, Spain aut Lamas Jesús Departamento de Biología Celular y Ecología, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut Lemos Manuel Departamento de Microbiología y Parasitología, Instituto de Acuicultura, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut Martínez Paulino Departamento de Genética, Facultad de Veterinaria, Campus de Lugo, Universidad de Santiago de Compostela, 27002, Lugo, Spain aut Marine Biotechnology Springer-Verlag 13/6(2011-12-01), 1099-1114 1436-2228 13:6<1099 2011 13 10126 https://doi.org/10.1007/s10126-011-9374-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-011-9374-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Millán Adrián Departamento de Genética, Facultad de Veterinaria, Campus de Lugo, Universidad de Santiago de Compostela, 27002, Lugo, Spain aut NATIONALLICENCE 700 1- Gómez-Tato Antonio Departamento de Geometría y Topología, Facultad de Matemáticas, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Pardo Belén Departamento de Genética, Facultad de Veterinaria, Campus de Lugo, Universidad de Santiago de Compostela, 27002, Lugo, Spain aut NATIONALLICENCE 700 1- Fernández Carlos Departamento de Genética, Facultad de Veterinaria, Campus de Lugo, Universidad de Santiago de Compostela, 27002, Lugo, Spain aut NATIONALLICENCE 700 1- Bouza Carmen Departamento de Genética, Facultad de Veterinaria, Campus de Lugo, Universidad de Santiago de Compostela, 27002, Lugo, Spain aut NATIONALLICENCE 700 1- Vera Manuel Departamento de Genética, Facultad de Veterinaria, Campus de Lugo, Universidad de Santiago de Compostela, 27002, Lugo, Spain aut NATIONALLICENCE 700 1- Alvarez-Dios José Departamento de Matemática Aplicada, Facultad de Matemáticas, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Cabaleiro Santiago Cluster de la Acuicultura de Galicia (CETGA), Punta de Couso s/n, Aguiño, 15965, A Coruña, Spain aut NATIONALLICENCE 700 1- Lamas Jesús Departamento de Biología Celular y Ecología, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Lemos Manuel Departamento de Microbiología y Parasitología, Instituto de Acuicultura, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Martínez Paulino Departamento de Genética, Facultad de Veterinaria, Campus de Lugo, Universidad de Santiago de Compostela, 27002, Lugo, Spain aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/6(2011-12-01), 1099-1114 1436-2228 13:6<1099 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer