caa a22 4500 445318147 CHVBK 20180317142654.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10126-011-9376-5 doi (NATIONALLICENCE)springer-10.1007/s10126-011-9376-5 Differential Growth-Related Gene Expression in Abalone ( Haliotis midae ) [Elektronische Daten] [Mathilde van der Merwe, Paolo Franchini, Rouvay Roodt-Wilding] The slow growth rate of Haliotis midae impedes the optimal commercial production of this most profitable South African aquaculture species. To date, no comprehensive effort has been made to identify genes associated with growth variation in farmed H. midae. The aim of this study was therefore to investigate growth variation in H. midae and to identify and quantify the expression of selected growth-related genes. Towards this aim, molecular methodologies and cell cultures were combined as a time-efficient and economical way of studying abalone transcriptomics and cell biology. Modern Illumina sequencing-by-synthesis technology and subsequent sequence annotation were used to elucidate differential gene expression between two sibling groups of abalone demonstrating significant growth variation. The expression of selected target genes involved in growth was subsequently analysed by quantitative real-time PCR (qPCR). Fast- and slow-growing abalone and in vitro primary haemocyte cultures treated with different growth-stimulating factors were used. The results obtained from transcriptome analysis and qPCR revealed significant differences in gene expression between large and small abalone, and between treated and untreated haemocyte cell cultures. Throughout in vivo and in vitro qPCR experiments, the up-regulation of genes involved in the insulin signalling pathway suggests that insulin may be involved in enhanced growth rate for various H. midae tissues. Springer Science+Business Media, LLC, 2011 Haliotis midae nationallicence Gene expression nationallicence Growth nationallicence Transcriptome sequence nationallicence qPCR nationallicence Haemocyte culture nationallicence van der Merwe Mathilde Molecular Aquatic Research Group, Department of Genetics, Stellenbosch University, Private Bag X1, 7602, Matieland, South Africa aut Franchini Paolo Molecular Aquatic Research Group, Department of Genetics, Stellenbosch University, Private Bag X1, 7602, Matieland, South Africa aut Roodt-Wilding Rouvay Molecular Aquatic Research Group, Department of Genetics, Stellenbosch University, Private Bag X1, 7602, Matieland, South Africa aut Marine Biotechnology Springer-Verlag 13/6(2011-12-01), 1125-1139 1436-2228 13:6<1125 2011 13 10126 https://doi.org/10.1007/s10126-011-9376-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-011-9376-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van der Merwe Mathilde Molecular Aquatic Research Group, Department of Genetics, Stellenbosch University, Private Bag X1, 7602, Matieland, South Africa aut NATIONALLICENCE 700 1- Franchini Paolo Molecular Aquatic Research Group, Department of Genetics, Stellenbosch University, Private Bag X1, 7602, Matieland, South Africa aut NATIONALLICENCE 700 1- Roodt-Wilding Rouvay Molecular Aquatic Research Group, Department of Genetics, Stellenbosch University, Private Bag X1, 7602, Matieland, South Africa aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/6(2011-12-01), 1125-1139 1436-2228 13:6<1125 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer