caa a22 4500 445318163 CHVBK 20180317142654.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10126-011-9370-y doi (NATIONALLICENCE)springer-10.1007/s10126-011-9370-y Effect of the Nutritional Status of Semi-continuous Microalgal Cultures on the Productivity and Biochemical Composition of Brachionus plicatilis [Elektronische Daten] [Martiña Ferreira, Pedro Seixas, Paula Coutinho, Jaime Fábregas, Ana Otero] The rotifer Brachionus plicatilis was cultured using the microalga Isochrysis aff. galbana clone T-ISO as feed. T-ISO was cultured semi-continuously with daily renewal rates of 10%, 20%, 30%, 40%, and 50% of the volume of cultures. The increase of renewal rate led to increasing nutrient and light availability in microalgal cultures, which caused differences in the biochemical composition of microalgal biomass. Growth rate, individual dry weight, organic content, and biomass productivity of rotifer cultures increased in response to higher growth rate in T-ISO cultures. Rotifer growth rate showed a strong negative correlation (R 2 = 0.90) with the C/N ratio of microalgal biomass. Rotifer dry weight was also affected by nutrient availability of T-ISO cultures, increasing up to 50% from nutrient-limited to nutrient-sufficient conditions. Consequently, biomass productivity of rotifer cultures increased more than twofold with the increase of renewal rate of T-ISO cultures. Rotifer organic content underwent the same trend of total dry weight. Maximum content of polyunsaturated fatty acids was reached in rotifers fed T-ISO from the renewal rate of 40%, with percentages of docosahexaenoic acid (22:6ω-3, DHA) and eicosapentaenoic acid (20:5ω-3, EPA) of 11% and 5% of total fatty acids, respectively. Selecting the most appropriate conditions for microalgal culture can therefore enhance the nutritive quality of microalgal biomass, resulting in a better performance of filter feeders and their nutrient content, and may constitute a useful tool to improve the rearing of fish larvae and other aquaculture organisms that require live feed in some or all the stages of their life cycle. Springer Science+Business Media, LLC, 2011 Brachionus plicatilis nationallicence Microalgae nationallicence Semi-continuous culture nationallicence Nutrition nationallicence Productivity nationallicence Biochemical composition nationallicence Ferreira Martiña Department of Microbiology and Parasitology, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut Seixas Pedro Department of Microbiology and Parasitology, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut Coutinho Paula Department of Microbiology and Parasitology, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut Fábregas Jaime Department of Microbiology and Parasitology, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut Otero Ana Department of Microbiology and Parasitology, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut Marine Biotechnology Springer-Verlag 13/6(2011-12-01), 1074-1085 1436-2228 13:6<1074 2011 13 10126 https://doi.org/10.1007/s10126-011-9370-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-011-9370-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ferreira Martiña Department of Microbiology and Parasitology, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Seixas Pedro Department of Microbiology and Parasitology, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Coutinho Paula Department of Microbiology and Parasitology, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Fábregas Jaime Department of Microbiology and Parasitology, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Otero Ana Department of Microbiology and Parasitology, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/6(2011-12-01), 1074-1085 1436-2228 13:6<1074 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer