caa a22 4500 445318171 CHVBK 20180317142654.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10126-011-9391-6 doi (NATIONALLICENCE)springer-10.1007/s10126-011-9391-6 Marine-Based Cultivation of Diacarnus Sponges and the Bacterial Community Composition of Wild and Maricultured Sponges and Their Larvae [Elektronische Daten] [Oded Bergman, Markus Haber, Boaz Mayzel, Matthew Anderson, Muki Shpigel, Russell Hill, Micha Ilan] Marine organisms including sponges (Porifera) contain many structurally diverse bioactive compounds, frequently in a low concentration that hampers their commercial production. Two solutions to this problem are: culturing sponge explants for harvesting the desired compound and cultivation of sponge-associated bacteria. These bacteria (often considered the source of the desired compounds) include the Actinobacteria, from which many novel drugs were developed. In a long-term experiment (lasting 767days), we evaluated the culture amenability of the sponge Diacarnus erythraenus in a mariculture system, placed at 10- and 20-m depths. The growth and survival rates of sponge fragments were monitored. Wild and maricultured sponges from both depths and their larvae were sampled at different time intervals for denaturing gradient gel electrophoresis (DGGE) profiling of the bacterial community residing within them. 16S rRNA gene sequences of both cultured bacterial isolates and clone libraries of unculturable bacteria were composed and compared, focusing on Actinobacteria. Sponges from both depths did not differ significantly either in mean growth rates (percent weight change year−1 ± S.E.) (64.5% ± 21% at 10m and 79.3% ± 19.1% at 20m) or in seasonal growth rates. Survival was also very similar (72% at 10m and 70% at 20m). There were 88 isolates identified from adults and 40 from their larvae. The isolates and clone libraries showed diverse bacterial communities. The DGGE profiles of wild and maricultured sponges differed only slightly, without a significant effect of depths or dates of sampling. This long-term experiment suggests that D. erythraenus probably remained healthy and indicates its mariculture suitability. Springer Science+Business Media, LLC, 2011 Diacarnus erythraenus nationallicence Red Sea nationallicence 16S rRNA gene nationallicence Aquaculture nationallicence Mariculture nationallicence Phylogenetic analysis nationallicence Marine natural products nationallicence Bergman Oded Department of Zoology, Tel Aviv University, 69978, Tel Aviv, Israel aut Haber Markus Department of Zoology, Tel Aviv University, 69978, Tel Aviv, Israel aut Mayzel Boaz Department of Zoology, Tel Aviv University, 69978, Tel Aviv, Israel aut Anderson Matthew Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Columbus Center, Suite 236, 701 East Pratt Street, 21202, Baltimore, MD, USA aut Shpigel Muki National Center for Mariculture, IOLR, P.O. Box1212, 88212, Eilat, Israel aut Hill Russell Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Columbus Center, Suite 236, 701 East Pratt Street, 21202, Baltimore, MD, USA aut Ilan Micha Department of Zoology, Tel Aviv University, 69978, Tel Aviv, Israel aut Marine Biotechnology Springer-Verlag 13/6(2011-12-01), 1169-1182 1436-2228 13:6<1169 2011 13 10126 https://doi.org/10.1007/s10126-011-9391-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-011-9391-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bergman Oded Department of Zoology, Tel Aviv University, 69978, Tel Aviv, Israel aut NATIONALLICENCE 700 1- Haber Markus Department of Zoology, Tel Aviv University, 69978, Tel Aviv, Israel aut NATIONALLICENCE 700 1- Mayzel Boaz Department of Zoology, Tel Aviv University, 69978, Tel Aviv, Israel aut NATIONALLICENCE 700 1- Anderson Matthew Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Columbus Center, Suite 236, 701 East Pratt Street, 21202, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Shpigel Muki National Center for Mariculture, IOLR, P.O. Box1212, 88212, Eilat, Israel aut NATIONALLICENCE 700 1- Hill Russell Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Columbus Center, Suite 236, 701 East Pratt Street, 21202, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Ilan Micha Department of Zoology, Tel Aviv University, 69978, Tel Aviv, Israel aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/6(2011-12-01), 1169-1182 1436-2228 13:6<1169 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer