caa a22 4500 44531818X CHVBK 20180317142654.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10126-011-9369-4 doi (NATIONALLICENCE)springer-10.1007/s10126-011-9369-4 Bioactivity, Chemical Profiling, and 16S rRNA-Based Phylogeny of Pseudoalteromonas Strains Collected on a Global Research Cruise [Elektronische Daten] [Nikolaj Vynne, Maria Månsson, Kristian Nielsen, Lone Gram] One hundred one antibacterial Pseudoalteromonas strains that inhibited growth of a Vibrio anguillarum test strain were collected on a global research cruise (Galathea 3), and 51 of the strains repeatedly demonstrated antibacterial activity. Here, we profile secondary metabolites of these strains to determine if particular compounds serve as strain or species markers and to determine if the secondary metabolite profile of one strain represents the bioactivity of the entire species. 16S rRNA gene similarity divided the strains into two primary groups: One group (51 strains) consisted of bacteria which retained antibacterial activity, 48 of which were pigmented, and another group (50 strains) of bacteria which lost antibacterial activity upon sub-culturing, two of which were pigmented. The group that retained antibacterial activity consisted of six clusters in which strains were identified as Pseudoalteromonas luteoviolacea, Pseudoalteromonas aurantia, Pseudoalteromonas phenolica, Pseudoalteromonas ruthenica, Pseudoalteromonas rubra, and Pseudoalteromonas piscicida. HPLC-UV/VIS analyses identified key peaks, such as violacein in P. luteoviolacea. Some compounds, such as a novel bromoalterochromide, were detected in several species. HPLC-UV/VIS detected systematic intra-species differences for some groups, and testing several strains of a species was required to determine these differences. The majority of non-antibacterial, non-pigmented strains were identified as Pseudoalteromonas agarivorans, and HPLC-UV/VIS did not further differentiate this group. Pseudoalteromonas retaining antibacterial were more likely to originate from biotic or abiotic surfaces in contrast to planktonic strains. Hence, the pigmented, antibacterial Pseudoalteromonas have a niche specificity, and sampling from marine biofilm environments is a strategy for isolating novel marine bacteria that produce antibacterial compounds. Springer Science+Business Media, LLC, 2011 Pseudoalteromonas nationallicence Antibacterial activity nationallicence Secondary metabolites nationallicence Bioprospecting nationallicence Galathea 3 nationallicence Vynne Nikolaj National Food Institute, Technical University of Denmark, Søltofts Plads, bldg. 221, 2800, Kgs. Lyngby, Denmark aut Månsson Maria Department of Systems Biology, Technical University of Denmark, Søltofts Plads, bldg. 221, 2800, Kgs. Lyngby, Denmark aut Nielsen Kristian Department of Systems Biology, Technical University of Denmark, Søltofts Plads, bldg. 221, 2800, Kgs. Lyngby, Denmark aut Gram Lone National Food Institute, Technical University of Denmark, Søltofts Plads, bldg. 221, 2800, Kgs. Lyngby, Denmark aut Marine Biotechnology Springer-Verlag 13/6(2011-12-01), 1062-1073 1436-2228 13:6<1062 2011 13 10126 https://doi.org/10.1007/s10126-011-9369-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-011-9369-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vynne Nikolaj National Food Institute, Technical University of Denmark, Søltofts Plads, bldg. 221, 2800, Kgs. Lyngby, Denmark aut NATIONALLICENCE 700 1- Månsson Maria Department of Systems Biology, Technical University of Denmark, Søltofts Plads, bldg. 221, 2800, Kgs. Lyngby, Denmark aut NATIONALLICENCE 700 1- Nielsen Kristian Department of Systems Biology, Technical University of Denmark, Søltofts Plads, bldg. 221, 2800, Kgs. Lyngby, Denmark aut NATIONALLICENCE 700 1- Gram Lone National Food Institute, Technical University of Denmark, Søltofts Plads, bldg. 221, 2800, Kgs. Lyngby, Denmark aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/6(2011-12-01), 1062-1073 1436-2228 13:6<1062 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer