caa a22 4500 445318198 CHVBK 20180317142654.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10126-011-9377-4 doi (NATIONALLICENCE)springer-10.1007/s10126-011-9377-4 Molecular Characterization of Adenosine 5′-monophosphate Deaminase—The Key Enzyme Responsible for the Umami Taste of Nori ( Porphyra yezoensis Ueda, Rhodophyta) [Elektronische Daten] [Seiko Minami, Minoru Sato, Yoshihiro Shiraiwa, Koji Iwamoto] The enzyme adenosine 5′-monophosphate deaminase (AMPD, EC 3.5.4.6) catalyzes the conversion of adenosine 5′-monophosphate to inosine 5′-mononucleotide (IMP). IMP is generally known as the compound responsible for the umami taste of the edible red alga Porphyra yezoensis Ueda that is known in Japan as nori. Therefore, we suspect that AMPD plays a key role in providing a favorable nori taste. In this study, we undertake the molecular characterization of nori-derived AMPD. The nori AMPD protein has a molecular mass of 55kDa as estimated from both gel filtration and sodium dodecyl sulfate polyacrylamide gel electrophoresis. The calculated molecular mass from the amino acid sequence deduced from cDNA is 57.1kDa. The isoelectric point is 5.71. The coding region of AMPD consists of 1,566bp encoding 522 amino acids and possesses a transmembrane domain and two N-glycosylation sites. The sequence identity of nori AMPD in human and yeast AMPDs was found to be less than 50% and 20% in DNA and amino acid sequences, respectively. Proline in the conserved motif of [SA]-[LIVM]-[NGS]-[STA]-D-D-P was found to be converted to glutamate. These results indicate that nori AMPD is a novel type of AMPD. Springer Science+Business Media, LLC, 2011 Umami nationallicence 5′-AMP deaminase (AMPD) nationallicence 5′-inosinic acid (IMP) nationallicence Nori nationallicence Porphyra yezoensis nationallicence Minami Seiko Graduate School of Agricultural Science, Tohoku University, Sendai, 985-8555, Miyagi, Japan aut Sato Minoru Graduate School of Agricultural Science, Tohoku University, Sendai, 985-8555, Miyagi, Japan aut Shiraiwa Yoshihiro Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8572, Ibaraki, Japan aut Iwamoto Koji Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8572, Ibaraki, Japan aut Marine Biotechnology Springer-Verlag 13/6(2011-12-01), 1140-1147 1436-2228 13:6<1140 2011 13 10126 https://doi.org/10.1007/s10126-011-9377-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-011-9377-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Minami Seiko Graduate School of Agricultural Science, Tohoku University, Sendai, 985-8555, Miyagi, Japan aut NATIONALLICENCE 700 1- Sato Minoru Graduate School of Agricultural Science, Tohoku University, Sendai, 985-8555, Miyagi, Japan aut NATIONALLICENCE 700 1- Shiraiwa Yoshihiro Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8572, Ibaraki, Japan aut NATIONALLICENCE 700 1- Iwamoto Koji Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8572, Ibaraki, Japan aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/6(2011-12-01), 1140-1147 1436-2228 13:6<1140 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer